非小细胞肺癌中PHF23,ACTN4表达与预后的相关性分析  

Correlation analysis of PHF23,ACTN4 expression and prognosis in non-small cell lung cancer

作　　者：葛建娟 白兆石 施向荣 周艳 谭清和[1] 李剑英 GE Jianjuan;BAI Zhaoshi;SHI Xiangrong;ZHOU Yan;TAN Qinghe;LI Jianying(Department of Medical Oncology,the Affiliated Cancer Hospital of Nantong University,Nantong,Jiangsu226001,China;Department of Pharmacy,Jiangsu Cancer Hospital,Nanjing,Jiangsu210009,China)

机构地区：[1]南通大学附属肿瘤医院肿瘤内科,江苏南通226001 [2]江苏省肿瘤医院药学部,江苏南京210009

出　　处：《临床肺科杂志》2024年第11期1712-1718,共7页Journal of Clinical Pulmonary Medicine

基　　金：江苏省卫生健康委医学科研项目(No.H2023002)。

摘　　要：目的研究非小细胞肺癌(NSCLC)中PHD锌指蛋白23(PHF23),α-辅肌动蛋白(ACTN4)的表达及预后的相关性。方法选取2017年2月至2020年11月期间我院收治的82例NSCLC患者。采用实时荧光定量PCR和免疫组化检测NSCLC组织及癌旁组织PHF23,ACTN4表达。相关性分析采用Spearman秩相关和Pearson相关分析。Kaplan-Meier曲线分析PHF23,ACTN4表达与患者预后的关系。Cox回归分析患者预后影响因素。结果NSCLC癌组织中PHF23,ACTN4表达阳性率分别为73.17%(60/82),75.61%(62/82),高于癌旁组织7.32%(6/82),6.10%(5/82),差异具有统计学意义(χ^(2)=73.937,81.987,均P<0.001)。NSCLC癌组织中PHF23与ACTN4表达呈正相关(r=0.745,P<0.001)。NSCLC癌组织中PHF23 mRNA,ACTN4 mRNA表达及侵袭转移指标N-钙黏素mRNA,波形蛋白mRNA高于癌旁组织,差异具有统计学意义(均P<0.05)。NSCLC癌组织中PHF23 mRNA与N-钙黏素mRNA,波形蛋白mRNA呈正相关(r=0.671,0.721,均P<0.001)。ACTN4 mRNA与N-钙黏素mRNA,波形蛋白mRNA呈正相关(r=0.663,0.708,均P<0.001)。肿瘤分期ⅢA期、淋巴结转移、在NSCLC癌组织中PHF23,ACTN4阳性率明显高于Ⅰ~Ⅱ期、无淋巴结转移的癌组织(P<0.05)。PHF23阳性组,ACTN4阳性组的NSCLC患者3年无进展生存率为25.00%(15/60),25.81%(16/62),分别低于PHF23阴性组,ACTN4阴性组81.82%(18/22),85.00%(17/20),差异具有统计学意义(Log Rankχ^(2)=17.810,26.380,均P<0.001)。TNM分期ⅢA期、淋巴结转移、PHF23阳性、ACTN4阳性是影响NSCLC患者预后的危险因素。结论NSCLC中PHF23,ACTN4表达上调,与TNM分期、淋巴结转移相关,是影响患者预后的独立危险因素。Objective To study the expression of PHD zinc finger protein 23(PHF23),α-actin 4(ACTN4)and their correlation with prognosis in non-small cell lung cancer(NSCLC).Methods 82 NSCLC patients admitted to our hospital from February 2017 to November 2020 were collected.Real time fluorescence quantitative PCR and immunohistochemistry were used to detect the expression of PHF23 and ACTN4 in NSCLC tissues and adjacent tissues.Correlation analysis was conducted by Spearman rank correlation and Pearson correlation analysis.Kaplan Meier curves were used to analyze the relationship between the expression of PHF23,ACTN4 in NSCLC tissue and prognosis.COX regression analysis was used to analyze prognostic factors in NSCLC patients.Conclusion The positive rate of PHF23 and ACTN4 expression in NSCLC cancer tissue was 73.17%(60/82)and 75.61%(62/82),respectively,which were significantly higher than those in adjacent tissues 7.32%(6/82)and 6.10%(5/82),with statistical significance(χ^(2)=73.937,81.987,P<0.001).The expression of PHF23 and ACTN4 in NSCLC tissues was positively correlated(r=0.745,P<0.001).The positive rate of PHF23 and ACTN4 in NSCLC cancer tissues with tumor stageⅢA and lymph node metastasis was significantly higher than those in stage Ⅰ-Ⅱcancer tissues without lymph node metastasis(P<0.05).The expression of PHF23 mRNA,ACTN4 mRNA,N-cadherin mRNA and vimentin mRNA were higher in NSCLC cancer tissues than in adjacent tissues(P<0.05).PHF23 mRNA was positively correlated with N-cadherin mRNA and vimentin mRNA in NSCLC cancer tissue(r=0.671,0.721,P<0.001).ACTN4 mRNA was positively correlated with N-cadherin mRNA and vimentin mRNA(r=0.663,0.708,P<0.001).The 3-year progression free survival rate of NSCLC patients in the PHF23 positive group and the ACTN4 positive group was 25.00%(15/60)and 25.81%(16/62),respectively,lower than that in the PHF23 negative group and the ACTN4 negative group,which was 81.82%(18/22)and 85.00%(17/20)(Log Rankχ^(2)=17.810,26.380,P<0.001).TNM stageⅢA,lymph node metastasis,positive PHF23,and

关 键 词：非小细胞肺癌 PHD锌指蛋白23 α-辅肌动蛋白 预后 

分 类 号：R734.2[医药卫生—肿瘤]