卵巢上皮性癌组织miR-143-3p、TIMELESS mRNA表达与患者临床病理特征及预后的关系  

Relationships of expression of miR-143-3p and TIMELESS mRNA in epithelial ovarian cancer tissues with clinicopathological characteristics and prognosis of patients

作　　者：吴芳芳 黄靖惠 余蕾 WU Fangfang;HUANG Jinghui;YU Lei(Clinical Medicine School,Guizhou Medical University,Guiyang 550000,China;不详)

机构地区：[1]贵州医科大学临床医学院,贵阳550000 [2]贵州医科大学附属妇女儿童医院贵阳市妇幼保健院中心实验室

出　　处：《山东医药》2024年第31期15-19,共5页Shandong Medical Journal

基　　金：贵州省卫生健康委员会基金项目(gzwjkj2020-2-106)。

摘　　要：目的探讨卵巢上皮性癌(EOC)组织微小核糖核酸-143-3p(miR-143-3p)、永恒昼夜节律调节器(TIME⁃LESS)mRNA表达与患者临床病理特征、预后的关系。方法选取EOC患者106例,术中收集EOC组织及对应癌旁组织,用实时荧光定量聚合酶链反应检测miR-143-3p、TIMELESS mRNA。通过TargetScan、StarBase、miRWalk等在线数据库预测miR-143-3p与TIMELESS的结合位点,Pearson相关法分析miR-143-3p与TIMELESS mRNA表达的相关性。分析miR-143-3p、TIMELESS mRNA表达与EOC患者临床病理特征的关系。对EOC患者随访3年,统计累积生存率,根据miR-143-3p、TIMELESS mRNA相对表达量均值,将患者分为miR-143-3p、TIMELESS mRNA高/低表达者,Kaplan-Meier法绘制生存曲线,进行生存分析;多因素Cox回归分析miR-143-3p、TIMELESS表达对EOC患者预后的影响。结果与癌旁组织比较,EOC组织中miR-143-3p相对表达量低,TIMELESS mRNA相对表达量高(P均<0.05)。miR-143-3p与TIMELESS在3′-非翻译区1453~1460处存在结合位点。EOC组织中miR-143-3p表达与TIMELESS mRNA表达呈负相关(r=-0.748,P<0.05)。miR-143-3p、TIMELESS mRNA表达与肿瘤国际妇产科联盟(FIGO)分期、分化程度、淋巴结转移和远处转移有关(P均<0.05)。随访3年,106例EOC患者3年累积生存率为69.81%(74/106)。miR-143-3p高表达者3年累积生存率高于miR-143-3p低表达者(P<0.05),TIMELESS mRNA高表达者3年累积生存率低于TIMELESS mRNA低表达者(P<0.05)。影响EOC患者预后的独立危险因素为FIGO分期Ⅲ~Ⅳ期、低分化、淋巴结转移、远处转移、TIMELESS mRNA≥1.11(P均<0.05),独立保护因素为miR-143-3p≥0.97(P<0.05)。结论EOC组织miR-143-3p低表达、TIMELESS mRNA高表达,二者表达变化与EOC的FIGO分期、分化程度、淋巴结转移、远处转移和预后密切相关。Objective To investigate the relationships of expression levels of microRNA-143-3p(miR-143-3p)and timeless circadian regulator(TIMELESS)mRNA in epithelial ovarian cancer(EOC)tissues with the clinicopathological characteristics and prognosis of patients.Methods We selected 106 patients with EOC,and the EOC tissues and corre⁃sponding adjacent tissues were collected.Real-time fluorescence quantitative PCR was performed to detect miR-143-3p and TIMELESS mRNA expression.The binding sites of miR-143-3p and TIMELESS were predicted by online databases such as TargetScan,StarBase,and miRWalk.Pearson correlation was used to analyze the correlation between miR-143-3p and TIMELESS mRNA.We analyzed the relationships between the expression of miR-143-3p and TIMELESS mRNA and the clinicopathological characteristics of EOC patients.EOC patients were followed up for three years,and we calculated the cumulative survival rate.EOC patients were divided into high/low miR-143-3p and TIMELESS mRNA expression groups according to the mean value of their expression,and Kaplan-Meier method was used to draw survival curves for sur⁃vival analysis.Multivariate Cox regression analysis was performed to analyze the effects of miR-143-3p and TIMELESS on the prognosis of EOC patients.Results Compared with the adjacent tissues,miR-143-3p was low expressed and TIME⁃LESS mRNA was highly expressed in EOC tissues(both P<0.05).The miR-143-3p and TIMELESS had a binding site at 3′-untranslated region 1453-1460.The miR-143-3p and TIMELESS mRNA expression in EOC tissues showed a negatively correlated(r=-0.748,P<0.05).The miR-143-3p and TIMELESS mRNA expression was related to International Federa⁃tion of Gynecology and Obstetrics(FIGO)stage,degree of differentiation,lymph node metastasis,and distant metastasis(all P<0.05).The 3-year overall survival rate of 106 EOC patients was 69.81%(74/106).The high miR-143-3p expres⁃sion patients had a higher 3-year overall survival rate than the low miR-143-3p expression patients,and the 3-year overall surviv

关 键 词：卵巢上皮性癌 微小核糖核酸-143-3p 永恒昼夜节律调节器 临床病理特征 预后 

分 类 号：R737.31[医药卫生—肿瘤]