使用响应面法优化酶动力学拆分(R/S)-δ-十四内酯  

Optimizing the Enzymatic Kinetic Resolution of(R/S)-δ-Tetradecalactone by Response Surface Methodology

作　　者：彭艳[1] 李春霓 龙苋魏 李俊澄 陆群[1] PENG Yan;LI Chun-ni;LONG Xian-wei;LI Jun-cheng;LU Qun(School of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China)

机构地区：[1]西南交通大学生命与工程学院,四川成都610031

出　　处：《分子催化（中英文）》2024年第5期418-426,I0001,I0002,共11页Journal of Molecular Catalysis(China)

基　　金：国家自然科学基金资助项目(2019G010129)。

摘　　要：采用柱前衍生HPLC分析方法,以δ-十四内酯为底物,对一系列脂肪酶进行了筛选,得到可特异性拆分底物为(R/S)-5-羟基十四烷酸的黑曲霉脂肪酶(ANL)和洋葱假单胞菌脂肪酶(PCL).考察了pH、温度、助溶剂、酶酯比和反应时间对产物光学纯度(ee_(p))、收率(Y)的影响.通过响应面分析方法(CCD)确定了最佳反应条件,优化后得到(S)-5-羟基十四烷酸(ee_(p1)≥99.9%,收率为42.0%)和(R)-5-羟基十四烷酸(ee_(p2)≥99.9%,收率达49.0%).该研究符合绿色化学要求,具有良好的应用潜力.A series of lipases were screened usingδ-tetradecalactone as substrate by pre-column derivatization HPLC analysis.Aspergillus niger lipase(ANL)and Pseudomonas cepacia lipase(PCL)with specific hydrolysis substrate of(R/S)-5-hydroxytetradecanoic acid were obtained.The effects of pH,temperature,cosolvent,enzymeester ratio and reaction time on the optical purity(ee_(p))and yield(Y)of the product were investigated.The optimum reaction conditions were determined by response surface analysis(CCD).After optimization,(S)-5-hydroxytetradecanoic acid(ee_(p1)≥99.9%,yield 42.0%)and(R)-5-hydroxytetradecanoic acid(ee_(p2)≥99.9%,yield 49.0%)were obtained.The system meets the requirements of green chemistry and has good application potential.

关 键 词：脂肪酶 特异性拆分 响应面法 羟基脂肪酸 

分 类 号：O643.32[理学—物理化学]