土壤中产纤维素酶菌株的筛选及鉴定  

Screening and Identification of Cellulase Strains in Soil

作　　者：韩文静 韩世昌 牛海鹏 乌力吉德力根 Han Wenjing;Han Shichang;Niu Haipeng;Wulijideligen(College of Chemistry and Life Science,Chifeng University,Chifeng 024000,China;Research Center of Fluid Machinery Engineering and Technology,Jiangsu University,Zhenjiang 212013,China)

机构地区：[1]赤峰学院化学与生命科学学院,内蒙古赤峰024000 [2]江苏大学流体机械工程技术研究中心,江苏镇江212013

出　　处：《黑龙江科学》2024年第20期97-100,共4页Heilongjiang Science

基　　金：2022年国家级与省级大学生创新创业训练计划项目“高产纤维素酶菌株助力乡村振兴”(202210138017);赤峰学院重点实验室建设项目(CFXYZD202001);2022内蒙古自治区科技计划项目“乳清酒加工工艺及其发酵剂优化研究与应用”(2022YFDZ0012)。

摘　　要：为筛选和鉴定土壤中产纤维素酶菌株,从赤峰学院农场土壤中五点采样,分离培养得到1株产纤维素酶菌株,对产酶菌株进行形态学观察及测序,确定该菌株为杰丁塞伯林德纳氏酵母(Cyberlindnera jadinii)。对菌株进行降解纤维素特性检验,将菌株接种到滤纸条崩解培养基中第7 d时能够将无菌滤纸条降解为白色糊状,表现了较好的纤维素降解能力,为进一步应用微生物降解纤维素提供了研究基础。In order to screen and identify cellulase strains in soil,the study cultures from five-point sampling collected from the farm soil of Chifeng University,isolates a cellulase producing strain,conducts morphological observation and sequencing on the cellulase producing strain,and identifies the strain as Cyberlindnera jadinii.The study tests cellulose degradation characteristics of the strain.The sterile filter paper strip can be degraded into white paste in the strain when inoculated into the filter paper strip disintegration medium on the 7th day,showing a good cellulose degradation ability,which provides a research basis for the further application of microbial degradation of cellulose.

关 键 词：纤维素酶 杰丁塞伯林德纳氏酵母 降解 

分 类 号：S714.3[农业科学—林学]