不动杆菌JW对苯甲酸与水杨酸的生物降解研究  

Biodegradation of Benzoate and Salicylate by Acinetobacter sp.JW

作　　者：马志远 封梦然 张旭旺 Ma Zhiyuan;Feng Mengran;Zhang Xuwang(PetroChina Huhhot Petrochemical Company;School of Ocean Science and Technology,Dalian University of Technology)

机构地区：[1]中国石油呼和浩特石化公司 [2]大连理工大学海洋科学与技术学院

出　　处：《油气田环境保护》2024年第5期38-44,共7页Environmental Protection of Oil & Gas Fields

摘　　要：苯甲酸与水杨酸是环境中常见的有机污染物,也是多种芳烃污染物降解的中间代谢物。文章考察了不动杆菌(Acinetobacter sp.)JW对苯甲酸与水杨酸的降解特性,结果表明JW能够以苯甲酸与水杨酸为唯一碳源生长,对浓度为50~500 mg/L苯甲酸与水杨酸的降解能力较强。反应体系pH值7.0对JW生长及降解苯甲酸与水杨酸更为适宜,而大部分金属离子对JW生长及污染物降解具有明显抑制作用。基于液相色谱-质谱联用分析与基因组注释,推测JW能够利用邻苯二酚途径与原儿茶酸途径降解苯甲酸,利用邻苯二酚途径降解水杨酸。Benzoic acid and salicylic acid are common organic pollutants in the environment and intermediate metabolites in the degradation of various aromatic hydrocarbon pollutants.This article examined the degradation characteristics of JW,a typical type of Acinetobacter sp.,on benzoic acid and salicylic acid.The results show that JW can grow using benzoic acid and salicylic acid as the sole carbon source,with a strong degradation capacity for concentrations ranging from 50 to 500 mg/L.A reaction system pH of 7.0 is more suitable for JW growth and the degradation of benzoic acid and salicylic acid.Most metal ions have a significant inhibitory effect on the growth of JW and the degradation of pollutants.Based on liquid chromatography-mass spectrometry(LC-MS)analysis and genome annotation,it is hypothesized that JW degrades benzoic acid via the catechol and protocatechuic acid pathways and degrades salicylic acid via the catechol pathway.

关 键 词：苯甲酸 水杨酸 生物降解 降解途径 不动杆菌 

分 类 号：X172[环境科学与工程—环境科学]