柴胡疏肝散对胃肠功能紊乱大鼠结肠肌层缝隙连接蛋白43表达的影响  

Effect of Chaihu Shugan Powder on Colonic Muscle Gap Connexin 43 Expression in Rats with Gastrointestinal Dysfunction

作　　者：彭雷[1] 程阔菊[2] 范才波[1] 朱丹 赵婷[1] 程俊 王娜 罗云[1] Peng Lei;Cheng Kuoju;Fan Caibo;Zhu Dan;Zhao Ting;Cheng Jun;Wang Na;Luo Yun(Department of Gastroenterology,Dazhou Hospital of Integrated Traditional Chinese and Western Medicine1,Dazhou 635000,Sichuan Province,China;Department of Pharmacy,Dazhou Hospital of Integrated Traditional Chinese and Western Medicine2,Dazhou 635000,Sichuan Province,China;Laboratory of Myocardial Electrophysiology,Southwest Medical University3,Luzhou 646000,Sichuan Province,China)

机构地区：[1]达州市中西医结合医院消化内科,四川达州635000 [2]达州市中西医结合医院药学部,四川达州635000 [3]西南医科大学心肌电生理学研究室,四川泸州646000

出　　处：《中国社区医师》2024年第29期3-5,共3页Chinese Community Doctors

基　　金：2015年四川省科技计划项目(编号:2015JY0270)。

摘　　要：目的:探讨柴胡疏肝散对胃肠功能紊乱大鼠结肠肌层缝隙连接蛋白43(CX43)表达的影响。方法:将8周龄SD大鼠100只随机分为对照(sham)组、模型(FGID)组、FGID+药物组及sham+药物组,每组25只。对于FGID组、FGID+药物组,利用腹腔注射利血平的方式建立胃肠功能紊乱大鼠模型;sham组及sham+药物组大鼠实施生理盐水腹腔注射;完成造模后,为sham+药物组及FGID+药物组大鼠实施柴胡疏肝散汤剂灌服,sham组与FGID组大鼠灌服灭菌注射用水;通过实时荧光定量聚合酶链反应、Westeron Blot及组织免疫荧光技术检测四组大鼠结肠肌层CX43 mRNA、CX43蛋白表达水平及CX43阳性表达率。结果:四组大鼠结肠肌层CX43 mRNA、CX43蛋白表达水平及CX43阳性表达率比较,差异无统计学意义(P>0.05)。结论:柴胡疏肝散能够提高胃肠功能紊乱大鼠结肠肌层CX43的表达水平,从而改善胃肠功能。Objective:To investigate the effect of Chaihu Shugan Powder on the expression of colonic muscle gap connexin 43(CX43)in rats with gastrointestinal dysfunction.Methods:One hundred SD rats aged eight weeks were randomly divided into control group(sham),model group(FGID),FGID+drug group and sham+drug group,with twenty-five rats in each group.For FGID group and FGID+drug group,the rat model of gastrointestinal dysfunction was established by intraperitoneal injection of reserpine.The rats in sham group and sham+drug group were injected with normal saline.After modeling,the rats in sham+drug group and FGID+drug group were given Chaihu Shugan powder decoction,and the rats in sham group and FGID group were given sterilization water for injection.CX43 mRNA and CX43 protein expression levels and CX43 positive expression rate were detected by real-time fluorescence quantitative polymerase chain reaction,Westeron Blot and tissue immunofluorescence.Results:The expression levels of CX43 mRNA and CX43 protein and the positive expression rate of CX43 in the colonic muscle of the four groups were compared,FGID group<FGID+drug group<Sham group and Sham+drug group,and the differences were statistically significant(P<0.05);there was no significant difference in CX43 mRNA expression between Sham group and Sham+drug group(P>0.05).Conclusion:Chaihu Shugan powder can improve the gastrointestinal function by increasing the superficial level of CX43 in colonic muscle layer in rats with gastrointestinal dysfunction.

关 键 词：柴胡疏肝散 缝隙连接蛋白43 胃肠功能紊乱 

分 类 号：R573.5[医药卫生—消化系统]