盐度胁迫下亚硝酸盐型同步脱氮除硫性能急性响应及功能菌群分析  

Acute response and functional microorganisms of simultaneous sulfide and nitrite removal processes under salinity stress

作　　者：肖景洪 陈碧龙 李雯 蔡靖[1] XIAO Jinghong;CHEN Bilong;LI Wen;CAI Jing(College of Environmental Science&Engineering,Zhejiang Gongshang University,Hangzhou 310018,China)

机构地区：[1]浙江工商大学环境科学与工程学院,浙江杭州310018

出　　处：《高校化学工程学报》2024年第5期823-830,共8页Journal of Chemical Engineering of Chinese Universities

摘　　要：针对生物处理高盐含氮废水过程中亚硝酸盐积累问题,因亚硝酸盐同步脱氮除硫工艺具有高基质耐受性和高基质去除性能,为探究该工艺对盐度(氯化钠的质量分数)胁迫的急性耐受性能,分别考察了盐度梯度和基质质量浓度对亚硝酸盐型同步脱氮除硫工艺性能的单一急性影响。升高基质质量浓度显著提高了基质去除速率(显著性值p<0.05),硫化物和亚硝酸盐中S和N的去除速率最高可达(88.7±0.57) mg·L^(-1)·h^(-1)和(5.51±0.21) mg·L^(-1)·h^(-1);而升高盐度则明显抑制了基质去除速率(p<0.01),硫化物和亚硝酸盐中S和N的去除速率降至(23.33±1.10) mg·L^(-1)·h^(-1)和(1.98±0.09) mg·L^(-1)·h^(-1)。并探究了不同盐度梯度和基质质量浓度联合作用下该工艺性能应激响应。当NaCl质量分数<2.4%时,适当升高基质质量浓度有助于提高硫化物去除速率,NaCl对硫化物去除速率的促进率分别为0.28(180 mg·L^(-1))和0.27(240 mg·L^(-1));而不论基质质量浓度如何,升高盐度皆使亚硝酸盐去除速率显著下降(p<0.01),且抑制率随基质质量浓度升高而增大。经高通量测序技术分析,该工艺优势功能菌属为sulfurovum、rhodobacteraceae、sulfurimonas和microbacterium,其相对丰度分别在28.55%~11.25%。Nitrite accumulation is problematic during biological treatment of high-salinity and nitrogen-rich wastewater,and simultaneous sulfide and nitrite removal(SSNR)processes demonstrate notable substrate tolerance and outstanding substrate removal performance.In order to explore acute tolerance to salinity stress,single effects of substrate concentration and salinity(mass fraction of NaCl)on the performance of SSNR were evaluated.The results show that increasing substrate concentration significantly elevated substrate removal rates(significance value p<0.05),as the maximum sulfide and nitrite removal rates reached(88.7±0.57)mg·L^(-1)·h^(-1) and(5.51±0.21)mg·L^(-1)·h^(-1),respectively.However,substrate removal rates were significantly inhibited by salinity(p<0.01),with sulfide and nitrite removal rates decreased to(23.33±1.10)mg·L^(-1)·h^(-1) and(1.98±0.09)mg·L^(-1)·h^(-1),respectively.Moreover,the combined effect of substrate concentration and salinity was explored.When the NaCl mass fraction was less than 2.4%,the sulfide removal rate was improved by rising substrate concentration,while the promotion rate of NaCl on sulfide removal rate were 0.28(180 mg·L^(-1))and 0.27(240 mg·L^(-1)),respectively.Nevertheless,nitrite removal rate dramatically decreased with rising salinity(p<0.01),while the salinity inhibition rate increased with rising substrate concentration.High-throughput sequencing technology shows that the dominant functional bacterial genera were sulfurovum,rhodobacteraceae,sulfurimonas and microbacterium with relative abundance of 28.55%-11.25%,respectively.

关 键 词：同步脱氮除硫工艺 盐度胁迫 基质质量浓度 双重胁迫 功能微生物 

分 类 号：X172[环境科学与工程—环境科学]