水通道蛋白4与小鼠脑血肿周围水肿的相关性研究  

Study on the correlation between aquaporin-4 and perihematomal edema in mice

作　　者：陈晶晶 李香 郭万亮[1] 王琪[1] 胡小庆 王希明[3] CHEN Jingjing;LI Xiang;GUO Wanliang;WANG Qi;HU Xiaoqing;WANG Ximing(Department of Radiology,Children's Hospital of Soochow University,Suzhou 215025,China;Department of Neurosurgery&Brain and Nerve Research Laboratory,the First Affiliated Hospital of Soochow University,Suzhou 215006,China;Department of Radiology,the First Affiliated Hospital of Soochow University,Suzhou 215006,China)

机构地区：[1]苏州大学附属儿童医院放射科,苏州215025 [2]苏州大学附属第一医院神经外科与脑神经研究实验室,苏州215006 [3]苏州大学附属第一医院放射科,苏州215006

出　　处：《中国研究型医院》2024年第5期28-33,共6页Chinese Research Hospitals

基　　金：江苏省重点学科建设项目(JSDW202242)。

摘　　要：目的 探讨小鼠脑出血(ICH)后血肿周围水肿(PHE)与水通道蛋白4(AQP4)极性变化的关系。方法 将18只C57BL/6小鼠通过简单随机分配法分为假手术组(Sham组)和脑出血后24 h组(ICH组),通过MR T_(2)加权成像(T_(2)WI)观察PHE的范围,用透射电子显微镜(TEM)观察星形胶质细胞足突的变化,利用蛋白质免疫印迹实验检测AQP4-M23的表达反映AQP4极化程度;同时,通过免疫荧光染色法评估AQP4与CD31(血管特异性标志物)的共定位情况揭示AQP4极化程度。使用GraphPad Prism 9.0软件进行统计分析,对符合正态分布及方差齐性的计量资料以x±s表示,两组之间的比较采用t检验,P<0.05为有统计学意义。结果 在T_(2)WI序列上,ICH组小鼠基底节区出现团片状低信号影,低信号周围出现不规则信号增高,即脑出血后PHE较为显著;TEM检查发现,PHE区脑组织内血管周围星形胶质细胞足突广泛肿胀;蛋白质免疫印迹实验,发现AQP4-M23表达降低,与Sham组相比差异具有统计学意义(t=3.892,P<0.01);PHE区脑组织的免疫荧光图像发现AQP4与血管标志物CD31的共定位显著减少,表明AQP4极化程度减弱。结论 脑出血后小鼠PHE形成与AQP4的极化程度减弱密切相关。Objective To investigate the relationship between perihematomal edema(PHE)and changes in the polarity of aquaporin-4(AQP4)following intracerebral hemorrhage(ICH)in mice.Methods Eighteen C57BL/6 mice were randomly divided into the sham-operation group(Sham group)and the 24-hour post-ICH group(ICH group).PHE was observed using MR T_(2)-weighted imaging(T_(2)WI).The changes in astrocytic endfeet were observed by transmission electron microscopy(TEM),and the expression of AQP4-M23 was detected by western blot to reflect the degree of AQP4 polarization.Additionally,immunofluorescence staining was used to assess the colocalization of AQP4 with CD31(a vascular-specific marker)to reveal the degree of AQP4 polarization.Statistical analysis was performed using GraphPad Prism 9.0 software.Quantitative data that met the normal distribution and homogeneity of variance are presented as x±s,and comparisons between the two groups were made using the t-test,with P<0.05 considered statistically significant.Results On the T_(2)WI sequence,the ICH group showed a patchy hypointense signal in the basal ganglia region,surrounded by an irregular hyperintense signal,indicating significant PHE after intracerebral hemorrhage.TEM examination revealed extensive swelling of astrocytic endfeet around blood vessels in the PHE region.Western blot analysis showed a significant reduction in AQP4-M23 expression compared to the Sham group(t=3.892,P<0.01).Immunofluorescence images of the brain tissue in the PHE region showed a significant reduction in the colocalization of AQP4 with the vascular marker CD31,indicating decreased AQP4 polarization.Conclusion The formation of PHE in mice following intracerebral hemorrhage is closely related to the decreased polarization of AQP4.

关 键 词：水通道蛋白4 星形细胞 脑出血 血肿周围水肿 

分 类 号：R743.34[医药卫生—神经病学与精神病学]