不同有机废弃物载体对Bacillus megaterium有效性的影响  

Effect of Different Organic Wastes as Carrier on Effectiveness of Bacillus megaterium

作　　者：刘丙花[1] 吴瑜健 刘方春[1] 丁延芹[2] 刘幸红[1] 马胜国 彭琳 于连家 马海林[1] LIU Binghua;WU Yujian;LIU Fangchun;DING Yanqin;LIU Xinghong;MA Shengguo;PENG Lin;YU Lianjia;MA Hailin(Institute of Resources and Environment,Shandong Academy of Forestry,Jinan 250014;College of Life Sciences,Shandong Agricultural University,Taian,Shandong 271018;Shandong Forestry Protection and Development Service Center,Jinan 250014)

机构地区：[1]山东省林业科学研究院资源与环境研究所,济南250014 [2]山东农业大学生命科学学院,山东泰安271018 [3]山东省林业保护和发展服务中心,济南250014

出　　处：《中国农学通报》2024年第30期55-60,共6页Chinese Agricultural Science Bulletin

基　　金：山东省重点研发计划(重大科技创新工程)项目“土壤结构改良微生物菌剂研发及生物强化关键技术研究”(2021CXGC010804)。

摘　　要：为了给Bacillus megaterium选择理想的吸附载体。测定了草炭、金针菇渣、腐熟鸡粪、牛粪、羊粪的吸菌量及其载体菌剂的有效活菌数、胞外多糖(EPS)含量和发芽指数。草炭的吸菌量最高为1.64 mL/g,但随存放时间延长,草炭载体菌剂的有效活菌数和EPS含量急剧降低,且38 d内的发芽指数均小于80%,表现植物毒性。鸡粪和金针菇渣的吸菌量虽小于草炭,但随存放时间延长,鸡粪载体菌剂的有效活菌数和EPS含量逐渐增大,金针菇渣载体菌剂的EPS含量逐渐增加、有效活菌数降低幅度缓慢,且存放7 d后发芽指数均大于80%,表现植物无毒性。第38天时鸡粪和金针菇渣载体菌剂的有效活菌数分别为1.29×10^(9)CFU/g和6.20×10^(8)CFU/g,EPS含量分别为535.29 mg/kg和559.34 mg/kg,均显著高于其他载体菌剂。鸡粪和金针菇渣是B.megaterium较为理想的吸附载体。In order to select the ideal carrier for Bacillus megaterium,grass charcoal,cow manure,sheep manure,chicken manure and enoki mushroom residue were used as carriers for B.megaterium to determine their effects on the adsorption capacity,effective viable bacteria number,exopolysaccharides(EPS)content and germination index of the bacterial solution.Charcoal had the highest bacterial liquid adsorption capacity which was 1.64 mL/g,but with the extension of storage time,the effective viable bacterial number and EPS content in the charcoal inoculum decreased sharply,and the germination index was less than 80%within 38 days,indicating the plant toxicity.Although the bacterial absorption capacity of chicken manure and enoki mushroom residue was smaller than that of grass charcoal,the effective viable bacterial number and EPS content in the chicken manure inoculum showed a trend of increasing.For the enoki mushroom residue inoculum,the EPS content increased gradually,and the effective viable bacteria number decreased slowly.The germination index of the two carrier inoculants was greater than 80%after 7 days of storage,indicating no plant toxicity.On the 38th day,the effective viable bacteria number of the chicken manure and enoki mushroom residue inoculants were 1.29×10^(9)CFU/g and 6.20×10^(8)CFU/g,respectively,the EPS content was 535.29 mg/kg and 559.34 mg/kg,respectively,which were significantly higher than other carrier inoculants.Chicken manure and enoki mushroom residue were the ideal carriers for B.megaterium.

关 键 词：吸附载体 吸附能力 有机废弃物 巨大芽孢杆菌 有效活菌数 发芽指数 胞外多糖 

分 类 号：S144.1[农业科学—肥料学]