工业大麻内生真菌HDM07挥发性成分分析  

Analysis of Volatile Components of Endophytic Fungus HDM07 in Cannabis sativa L.

作　　者：邢翔宇 丛添玥 关昕 祁可香 李宛儒 张赫 尤梦瑶 吴桐[2] 郑春英[1] XING Xiangyu;CONG Tianyue;GUAN Xin;QI Kexiang;LI Wanru;ZHANG He;YOU Mengyao;WU Tong;ZHENG Chunying(Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education&Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region&Key Laboratory of Microbiology,College of Heilongjiang Province&School of Life Sciences,Heilongjiang University,Harbin 150080;Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education&Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region&Key Laboratory of Molecular Biology,College of Heilongjiang Province&School of Life Sciences,Heilongjiang University,Harbin 150080)

机构地区：[1]黑龙江大学,生命科学学院,农业微生物技术教育部工程研究中心,黑龙江省寒区植物基因与生物发酵重点实验室,黑龙江省普通高校微生物重点实验室,哈尔滨150080 [2]黑龙江大学,生命科学学院,农业微生物技术教育部工程研究中心,黑龙江省寒区植物基因与生物发酵重点实验室,黑龙江省普通高校分子生物学重点实验室,哈尔滨150080

出　　处：《中国农学通报》2024年第30期103-113,共11页Chinese Agricultural Science Bulletin

基　　金：黑龙江省种业创新发展资金项目“寒地龙药重点中药材种子种苗DNA指纹图谱库、特征库及质量控制体系研发与推广”(NO.20230202);黑龙江省现代农业产业技术协同创新体系项目“麻类食品与药物研发协同创新项目”(YYM19STX-1);黑龙江大学横向项目“工业大麻内生真菌及其次生代谢物提取、分离、鉴定”(NO.21192);黑龙江大学横向项目“一株产大麻酚的大麻内生真菌及其应用”(NO.23114);黑龙江大学横向项目“一种利用甘草内生真菌发酵工业大麻的方法”(NO.23113)。

摘　　要：旨在开发利用工业大麻内生真菌HDM07挥发性成分,探索工业大麻内生真菌HDM07次生代谢产物生物合成途径。以工业大麻内生真菌HDM07为研究对象,采用GC-MS法和检索质谱数据库分析鉴定内生真菌HDM07发酵液和菌丝体中的挥发性成分,采用形态学观察法和18S rDNA序列分析法鉴定菌种。结果表明,经GC-MS法和质谱数据库检索分析,从内生真菌HDM07发酵液中鉴定出11种挥发性成分,在内生真菌HDM07菌丝体中鉴定出14种挥发性成分,工业大麻叶中鉴定了12种成分,空白对照品溶液(PDB培养基)中鉴定了7种成分;经鉴定内生真菌HDM07为链格孢菌(Alternaria alternate)。上述结果表明,HDM07发酵液挥发性成分与菌丝体中挥发性成分两者的合成途径既有联系又有区别。内生真菌HDM07的发酵液及菌丝体中均含有与工业大麻相同或相似的挥发性成分,也含有其特有的挥发性成分,说明该菌株与宿主植物工业大麻在合成挥发性成分方面具有一定的相关性。This study aims to exploit volatile components of endophytic fungus HDM07 isolated from Cannabis sativa L.,and to explore the biosynthetic pathway of secondary metabolites from HDM07.The research took the endophytic fungus HDM07 obtained from Cannabis sativa L.as the subject.GC-MS and retrieval mass spectrometry database were employed to identify the volatile components in both the fermentation broth and mycelium of HDM07.Morphological observation and 18S rDNA sequence analysis were conducted for strain identification.Results indicated that the method of GC-MS and mass spectrometry analysis,along with database retrieval from endophytic fungi HDM07,determined 11 volatile components in the fermented liquid.Furthermore,fungus HDM07 revealed 14 volatile constituents in mycelium and Cannabis sativa L.leaf identified 12 elements.In a blank reference substance solution(PDB medium),appraisal revealed 7 ingredients.The endophytic fungus HDM07 was identified as Alternaria alternate.These findings suggest that the synthesis pathways of volatile components in HDM07 fermentation broth and mycelia are both related yet distinct.Both the fermentation broth and mycelium of endophytic fungus HDM07 contained similar or identical volatile components to those found in Cannabis sativa L.,and also contained unique ones,indicating a certain correlation between the strain and its host plant's synthesis of volatile components.

关 键 词：工业大麻 工业大麻内生真菌HDM07 18S rDNA序列分析 气相色谱质谱法 挥发性成分 

分 类 号：S182[农业科学—农业基础科学]