益气聪明汤在光学离焦性近视大鼠巩膜组织MMP-2、p-JNK表达中的作用  

Effects of Yiqi Congming Decoction on the levels of matrix metalloproteinase-2 and phosphorylated c-Jun N-terminal kinase in the scleral tissues of rats with lens-induced myopia

作　　者：张鑫鑫 姜艳华[2] 赵磊[3] ZHANG Xinxin;JIANG Yanhua;ZHAO Lei(Liaoning University of Traditional Chinese Medicine,Shenyang 110032,Liaoning Province,China;Department of Ophthalmology,the Fourth People’s Hospital of Shengyang,China Medical University,Shenyang 110031,Liaoning Province,China;Department of Ophthalmology,the Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110034,Liaoning Province,China)

机构地区：[1]辽宁中医药大学,辽宁省沈阳市110032 [2]中国医科大学沈阳市第四人民医院眼科,辽宁省沈阳市110031 [3]辽宁中医药大学附属第二医院眼科,辽宁省沈阳市110034

出　　处：《眼科新进展》2024年第11期846-851,共6页Recent Advances in Ophthalmology

基　　金：国家自然科学基金项目(编号:82104714);辽宁省自然科学基金项目(编号:20170540557);辽宁省博士科研启动基金计划项目(编号:2022-BS-069)。

摘　　要：目的探究中药方剂益气聪明汤在光学离焦性近视(LIM)大鼠巩膜组织基质金属蛋白酶2(MMP-2)、磷酸化氨基末端蛋白激酶(p-JNK)表达中的作用。方法选取SPF级健康无眼疾21 d龄SD大鼠60只,所有大鼠均选择右眼为实验眼。雌雄不拘,随机分为空白(BG)组、模型(MG)组、中药低剂量(LG)组、中药中剂量(MD)组、中药高剂量(HG)组以及西药(WG)组,每组各10只。将-5 D近视离焦透镜打磨至直径约1.7 cm的圆形,在两侧打一小孔,分别缝于MG、LG、MD、HG、WG组大鼠右眼上下眼睑皮肤处建立LIM模型。在造模4周后开始灌胃,WG组大鼠用0.1 g·L^(-1)阿托品滴眼液滴眼,BG组大鼠不做任何干预,LG、MD、HG组给予益气聪明汤灌胃,MG、BG组给予生理盐水灌胃。记录每组大鼠造模前、造模4周及8周后的眼轴长度。造模8周后处死大鼠,苏木素-伊红(HE)染色观察各组大鼠巩膜组织形态学变化,Western blot检测各组大鼠巩膜组织中MMP-2、p-JNK蛋白表达,免疫组织化学检测各组大鼠巩膜组织中MMP-2阳性表达,透射电镜观察各组大鼠巩膜胶原纤维直径大小。结果造模4周和8周后,与BG组比较,MG组大鼠眼轴长度均明显增加(均为P<0.001);与MG组比较,WG、LG、MD、HG组大鼠眼轴长度均减小(均为P<0.05)。造模8周后,BG大鼠的巩膜层间胶原纤维排列规则,走行正常;与BG组比较,MG组大鼠巩膜胶原纤维疏松有明显空隙,排列紊乱;与MG组比较,MD、HG组大鼠巩膜胶原纤维排列较规则,纤维间空隙变小,LG、WG组大鼠巩膜各层间胶原纤维相对清晰紧实。造模8周后,与BG组比较,MG组大鼠巩膜中MMP-2、p-JNK蛋白相对表达水平均明显升高(均为P<0.001);与MG组比较,WG、LG、MD、HG组大鼠巩膜中MMP-2、p-JNK蛋白相对表达水平均降低(均为P<0.05)。造模8周后,与BG组比较,MG组大鼠巩膜中MMP-2蛋白阳性表达升高(P<0.001);与MG组比较,WG、LG、MD、HG组大鼠巩膜中MMP-2蛋白阳性表达均降�Objective To investigate the effects of Chinese medicine prescription Yiqi Congming Decoction on the expressions of matrix metalloproteinase-2(MMP-2)and phosphorylated c-Jun N-terminal kinase(p-JNK)in the scleral tissues of rats with lens-induced myopia(LIM).Methods A total of 60 male and female specific-pathogen-free 21d Sprague Dawley rats without eye disease were included.The right eye was selected.These rats were randomly divided into the blank(BG)group,model(MG)group,low-dose traditional Chinese medicine(LG)group,medium-dose traditional Chinese medicine(MD)group,high-dose traditional Chinese medicine(HG)group and western medicine(WG)group,with 10 rats in each group.The-5 D myopic defocus lens was polished to a circle with a diameter of about 1.7 cm with small holes made on both sides and sewed on the upper and lower eyelid skin of the right eye of rats in the MG,LG,MD,HG and WG groups to build a LIM model.Intragastric administration was initiated 4 weeks after molding.Rats in the WG group were given 0.1 g·L^(-1) atropine eye drops,rats in the BG group did not receive any intervention,rats in the LG,MD and HG groups were administered with Yiqi Congming Decoction intragastrically,and rats in the MG and BG groups were given normal saline by intragastric administration.The axial length of rats in each group was recorded before,4 weeks and 8 weeks after modeling.The rats were sacrificed after 8 weeks of modeling.Hematoxylin-eosin(HE)staining was used to observe the histomorphological changes of sclera in rats.The protein expressions of MMP-2 and p-JNK in the sclera of rats were detected by Western blot.The positive expression of MMP-2 in the scleral tissues of rats was detected through immunohistochemistry.The diameter of the scleral collagen fibers of rats in each group was observed by a transmission electron microscope.Results At 4 weeks and 8 weeks after modeling,compared with the BG group,the axial length of rats in the MG group significantly increased(both P<0.001);compared with the MG group,the axial lengt

关 键 词：光学离焦性近视 巩膜 基质金属蛋白酶2 磷酸化氨基末端蛋白激酶 

分 类 号：R778.1[医药卫生—眼科]