高尿酸环境对晶状体透明度的影响及作用机制  

Impact of high uric acid environment on transparency of lens and its related mechanisms

作　　者：王盛 林宏亮 陈艳蕾 覃泳杰 张洪洋 WANG Sheng;LIN Hongliang;CHEN Yanlei;QIN Yongjie;ZHANG Hongyang(School of Medicine,South China University of Technology,Guangzhou 510006,Guangdong Province,China;Guangdong Provincial People’s Hospital,Guangdong Academy of Medical Sciences,Guangzhou 510080,Guangdong Province,China)

机构地区：[1]华南理工大学医学院,广东省广州市510006 [2]广东省人民医院,广东省医学科学院,广东省广州市510080

出　　处：《眼科新进展》2024年第11期852-856,共5页Recent Advances in Ophthalmology

基　　金：国家自然科学基金项目(编号:82171036);广东省中医药局科研项目(编号:20231012)。

摘　　要：目的通过构建大鼠晶状体离体培养模型探讨高尿酸环境对白内障形成的影响及机制。方法采用成年Sprague Dawley大鼠晶状体,在高尿酸培养环境下离体培养。将大鼠晶状体分为空白对照组(15眼)和尿酸干预组(15眼)。尿酸干预组采用含尿酸800μmol·L^(-1)的M-199培养液培养,空白对照组采用不含尿酸的M-199培养液培养,均培养7 d。通过光学显微镜观察两组大鼠晶状体混浊程度;Gomori六胺银法检测两组大鼠晶状体中尿酸盐沉积情况;SA-β-gal染色法检测两组大鼠晶状体细胞衰老情况;TUNEL染色法检测两组大鼠晶状体细胞凋亡情况。结果尿酸干预组大鼠晶状体于第5天时开始出现混浊,并于第7天时混浊分级显著升高,与空白对照组大鼠晶状体混浊分级相比,差异均有统计学意义(均为P<0.05)。空白对照组大鼠晶状体上皮细胞排列整齐,周围未见尿酸盐沉积,仅有极少数蓝染的晶状体上皮细胞,且未发现明显的阳性细胞;尿酸干预组大鼠晶状体赤道部及前囊下晶状体上皮细胞排列紊乱,周围出现黑棕色的尿酸盐结晶,出现较多的蓝染晶状体上皮细胞,且出现了少量阳性细胞。高尿酸环境下,尿酸盐结晶在大鼠晶状体囊膜下沉积,周围晶状体上皮细胞衰老,少量晶状体上皮细胞凋亡。结论高尿酸环境在大鼠晶状体离体培养模型中能够稳定有效地诱导白内障的发生,其机制可能与尿酸诱导晶状体上皮细胞发生衰老和凋亡有关。Objective To investigate the effect and mechanism of a high uric acid environment on cataract formation by rat lens ex-vivo culture model.Methods The lenses harvested from adult Sprague Dawley rats were cultured in a high uric acid environment in vitro.The lenses were divided into the blank control group(15 eyes)and the uric acid treatment group(15 eyes).The lenses in the uric acid treatment group were treated with M-199 medium with 800μmol·L^(-1) uric acid and lenses in the blank control group were cultured with M-199 medium without uric acid for 7 days.The turbidity of the lenses of rats in the two groups was observed by optical microscope.The deposition of urate in the lenses of rats in the two groups was detected by the Gomori hexamine silver method.The senescence of lens cells in the two groups was measured by SA-β-gal staining.Lens cell apoptosis in the two groups was detected by the TUNEL method.Results The opacification in lenses of the uric acid treatment group occurred on the 5th day and the opacification grade significantly increased on the 7th day,with opacification grades being significantly different from those in the blank control group(both P<0.05).In the blank control group,the lens epithelial cells were arranged neatly with no urate deposition,a few blue-stained lens epithelial cells and no obvious positive cells.In the uric acid treatment group,the arrangement of the lens epithelial was disordered in the equatorial part of the lens and under the anterior capsule,with black-brown urate crystals around the lens,more blue-stained lens epithelial cells and a small number of positive cells.In the high uric acid environment,urate crystals deposited under the capsular membrane of the lens,the surrounding lens epithelial cells were senescent,and a small number of lens epithelial cells died.Conclusion A high uric acid environment can steadily and effectively induce cataract formation in the rat lens ex-vivo culture model.The mechanism may be related to senescence and apoptosis of lens epithelial cell

关 键 词：白内障 晶状体上皮细胞 离体培养 尿酸 衰老 凋亡 

分 类 号：R776.1[医药卫生—眼科]