芽孢杆菌应用方式降低烟叶烟草特有亚硝胺含量的研究  

Reduction of TSNAs Content in Tobacco Leaves Using Bacillus Application Methods

作　　者：李振杰 邹玲 刘春波 何沛 韦克毅 赵英良 胡彬彬 刘志华 LI Zhenjie;ZOU Ling;LIU Chunbo;HE Pei;WEI Keyi;ZHAO Yingliang;HU Binbin;LIU Zhihua(Yunnan Key Laboratory of Tobacco Chemistry,R&D Center of China Tobacco Yunnan Industry Co.,Ltd.,Kunming 650231,China;Yunnan Academy of Tobacco Agricultural Sciences,Kunming 650021,China;Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]云南中烟工业有限责任公司技术中心,云南省烟草化学重点实验室,云南昆明650231 [2]云南省烟草农业科学研究院,云南昆明650021 [3]昆明理工大学生命科学与技术学院,云南昆明650500

出　　处：《云南农业大学学报（自然科学版）》2024年第5期48-54,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省烟草化学重点实验室开放课题(2020539200340214);云南中烟技术中心科技项目(11300295)。

摘　　要：【目的】研究短小芽孢杆菌05-5402和高地芽孢杆菌J54应用方式对烟叶中烟草特有亚硝胺(tobaccospecific nitrosamines,TSNAs)含量的影响。【方法】将TSNAs降解菌种施加于生长中的白肋烟和旱烟,采收成熟的烟叶,晾制后检测烟叶中的TSNAs含量;利用菌种发酵烘烤后的烟叶,分析不同发酵方式对烤烟烟叶中TSNAs含量的影响。【结果】(1)在晾晒烟的生长过程中,施加芽孢杆菌菌液能极显著降低烟叶TSNAs的含量,其中,05-5402对白肋烟TSNAs的降解效率较高,为54.75%~60.78%;而J54对旱烟TSNAs的降解效率较高,为53.23%~63.18%。(2)与固态发酵相比,液态发酵模式下菌株降解TSNAs的效果更好,05-5402和J54分别能显著降低烟叶中31.99%和35.86%的TSNAs。【结论】短小芽孢杆菌05-5402和高地芽孢杆菌J54能有效降低烟叶的TSNAs含量,并且在多种处理方式下均有较好的效果。[Purpose]To study the effects of application methods of Bacillus pumilus 05-5402 and B.altitudinis J54 on the content of tobacco-specific nitrosamines(TSNAs)in tobacco leaves.[Methods]The TSNAs-degrading strains were applied to the growing burley and Han tobacco plants,and the mature tobacco leaves were harvested and dried to detect the TSNAs content.The flue-cured tobacco was further fermented with the strains,and the effects of different fermentation methods on the TSNAs content in tobacco leaves were analyzed.[Results]1)During the growth period of sun-cured tobacco,the content of TSNAs in tobacco leaves could be extremely significantly reduced by the application of Bacillus strains.Among them,05-5402 exhibited a higher degradation efficiency of TSNAs in burley tobacco,ranging from 54.75%to 60.78%;while J54 exhibited a higher degradation efficiency of TSNAs in Han tobacco,ranging from 53.23%to 63.18%.2)Compared with solid-state fermentation,liquid-state fermentation was more efficient in degrading TSNAs.05-5402 could significantly reduce 31.99%of TSNAs,and J54 could significantly reduce 35.86%of TSNAs.[Conclusion]B.pumilus 05-5402 and B.altitudinis J54 can effectively reduce the TSNAs content in tobacco leaves,and they are effective under various treatment methods.

关 键 词：芽孢杆菌 烟草特有亚硝胺 烟草 发酵 

分 类 号：S572.01[农业科学—烟草工业]