逆境与赤霉素调控下茶树BRX同源基因的系统性鉴定与响应模式  

Systematic identification and response patterns of BRX orthologs from Camellia sinensis under stress and gibberellin regulation

作　　者：陈益 杨妮 罗微 李静文 刘慧[2] 庄静 CHEN Yi;YANG Ni;LUO Wei;LI Jingwen;LIU Hui;ZHUANG Jing(Ministry of Agriculture and Rural Affair Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China,Tea Research Institution,College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;State Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学园艺学院,茶叶科学研究所,农业农村部华东地区园艺作物生物学与种质创制重点实验室,南京210095 [2]南京农业大学作物遗传与种质创新利用全国重点实验室,南京210095

出　　处：《西北植物学报》2024年第11期1725-1734,共10页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目(31870681);江苏省政策引导类计划项目(SZ-LYG202126);江苏园艺种质资源保护和创新计划(JS-FEM-202212);江苏高校优势学科建设项目(PAPD)。

摘　　要：【目的】鉴定茶树BRX家族基因,分析其在不同组织和逆境下的表达模式,为探究BRX基因功能奠定基础。【方法】基于茶树基因组数据库及TBtools等软件对BRX基因进行鉴定分析;以‘舒茶早’为材料,对其进行不同时间的盐、干旱、高温、低温及外源GA3处理;用RT-qPCR检测CsBRXs基因在不同组织和处理下的转录水平。【结果】共鉴定到5个CsBRXs基因,其编码蛋白由313~370个氨基酸组成,该家族基因结构相似且高度保守,启动子区域含激素和逆境响应元件,根据进化关系BRX蛋白被分为4个亚族。CsBRX1、CsBRX3和CsBRX5在嫩叶中均有表达,CsBRX4仅在老叶中表达;盐胁迫12 h时,CsBRX1、CsBRX2和CsBRX4的转录水平最高;Cs-BRX2快速响应低温胁迫,在1 h时表达量达峰值;CsBRX2对外源GA信号积极响应,在24 h时表达量最高。【结论】CsBRXs基因在茶树中的表达具有组织特异性,并差异化响应多种逆境胁迫和GA信号,为研究其抗逆机制提供指导。[Objective]Identification and characterization of the BRX family genes in tea plants,along with analysis of their expression patterns in various tissues and under stress conditions.The research lays the groundwork for investigation into the functions of the tea plant BRX genes.[Methods]Using the tea plant genome database and bioinformatics softwares such as TBtools,we identified and analyzed the BRX genes with the tea plant variety‘Shuchazao’.Different treatments,including salt,drought,high temperature,and low temperature stress,as well as exogenous GA3 application,were applied for various durations.The relative transcript abundance of the BRX gene family in different tissues and under stress conditions was quantified using RT-qPCR.[Results]A total of 5 CsBRX genes was identified,encoding proteins com-posed of 313-370 amino acids.The structure of genes was similar and highly conserved.The promoter regions contained hormone and stress response elements.Based on evolutionary relationships,BRX pro-teins were divided into 4 subfamilies.CsBRX1,CsBRX3,and CsBRX5 were all expressed in young leav-es,while CsBRX4 was exclusively expressed in old leaves.Under salt stress for 12 h,the transcriptional levels of CsBRX1,CsBRX2,and CsBRX4 were elevated to the greatest extend.CsBRX2 exhibited a swift response to low temperature stress,with its relative expression peaked at 1 h.CsBRX2 demonstra-ted a positive response to exogenous GA signaling,and its expression reached a peak at 24 h interval.[Conclusion]The CsBRX genes demonstrate tissue-specific expression patterns,responding variably to multiple stresses and GA signal,which offers insights into stress tolerance mechanisms of tea plants.

关 键 词：茶树 BRX基因家族 生物信息学 组织特异性 非生物胁迫 表达分析 

分 类 号：Q943.2[生物学—植物学] S571.1[农业科学—茶叶生产加工]