Cu_(7)Zn_(2)双金属簇整体柱制备及其液相色谱分离性能研究  

The preparation and separation application of Cu_(7)Zn_(2)bimetallic cluster monolithic column for liquid chromatography

作　　者：金什琦 褚琪琪 陈怀侠[1] JIN Shiqi;CHU Qiqi;CHEN Huaixia(College of Chemistry and Chemical Engineering,Hubei University,Wuhan 430062,China)

机构地区：[1]湖北大学化学化工学院,湖北武汉430062

出　　处：《湖北大学学报（自然科学版）》2024年第6期855-863,共9页Journal of Hubei University：Natural Science

基　　金：国家自然科学基金(21804034)资助。

摘　　要：在高效液相色谱不锈钢分离柱(150 mm×4.6 mm)内制备了Cu_(7)Zn_(2)双金属簇共聚整体柱,并考察了其分离性能。分别选择邻苯二甲酸酯类和多环芳烃为分析物,对Cu_(7)Zn_(2)双金属簇色谱柱进行分离性能研究和吸附机理探讨。结果表明,Cu_(7)Zn_(2)双金属簇整体柱与商品化C 18色谱柱相比分离速度更快,10 min内即可实现分析物的快速分离。同时Cu_(7)Zn_(2)双金属簇整体柱具有反相色谱柱的分离特点,吸附机理推测为疏水作用和π-π堆积作用。针间(n=10)、日间(n=5)和柱间(n=3)保留时间的重复性(RSDs)在0.8%~1.9%范围内,峰面积的重复性(RSDs)为1.3%~3.9%。因此,Cu_(7)Zn_(2)双金属簇共聚整体柱具有良好的高效液相色谱分离重复性和稳定性。Cu_(7)Zn_(2)bimetallic cluster copolymer monolith was prepared in the stainless steel separation column(150 mm×4.6 mm)and applied for liquid chromatographic separation.Phthalates and polycyclic aromatic hydrocarbons were selected as analytes to study the separation performance and adsorption mechanism of the Cu_(7)Zn_(2)chromatographic column.The results showed that the Cu_(7)Zn_(2)monolithic column had faster separation ability than the commercial C 18 chromatographic column,and the analytes were rapid separation within 10 min.The Cu_(7)Zn_(2)copolymerized monolithic column had the separation characteristics of a reverse phase chromatographic column,the adsorption mechanism was speculated to be hydrophobic interaction andπ-πinteraction.The repeatability(RSDs)of retention time and peak area of inter needle(n=10),inter day(n=5)and inter column(n=3)were in the range of 0.8%-1.9%and 1.3%-3.9%,respectively,which indicated that the Cu_(7)Zn_(2)bimetallic cluster copolymer monolith had good repeatability and stability.

关 键 词：Cu_(7)Zn_(2)双金属簇 共聚整体柱 色谱柱 

分 类 号：O657[理学—分析化学]