中西药联用对黑斑蛙源“歪头病”病原菌的体外抑菌效果研究  

The in vitro anti-bacterial effect of Chinese herbal medicines and antibioticsand their compounds on pathogens caused torticollis in black-spotted frogs

作　　者：黄美平 汪鑫 梅凯华 袁庆云 陈展鹏 熊勇 曹叙洲 万晓玲 陈楠 HUANG Meiping;WANG Xin;MEI Kaihua;YUAN Qingyun;CHEN Zhanpeng;XIONG Yong;CAO Xuzhou;WAN Xiaoling;CHEN Nan(School of Animal Science and Nutritional Engineering,Wuhan Polytechnic University,Wuhan,430023,China;Huanggang Academy of Agricultural Sciences,Huanggang 438000,China;Hubei Long Run Agricultural Technology Development Co.,Ltd.,Huanggang 438000,China)

机构地区：[1]武汉轻工大学动物科学与营养工程学院,武汉430023 [2]黄冈市农业科学院,黄冈438000 [3]湖北隆润农业科技发展有限公司,黄冈438000

出　　处：《武汉轻工大学学报》2024年第5期1-9,共9页Journal of Wuhan Polytechnic University

基　　金：湖北省自然科学基金青年项目(编号:2023AFB031),武汉市农科院创新体系科技强链项目(编号:DSQ202304);黄冈市2023年度青年拔尖人才培养计划(编号:2023HQB06),武汉轻工大学博士启动项目(编号:2024RZ047);武汉轻工大学校级“大学生创新创业训练计划项目”(编号202310496092)。

摘　　要：为研究常用抗生素和中草药对黑斑蛙源“歪头病”病原菌米尔伊丽莎白菌和肺炎克雷伯菌的体外抑菌效果,采用纸片扩散法和二倍稀释法测定了米尔伊丽莎白菌和肺炎克雷伯菌对9种常用抗生素和6种中草药的敏感性,棋盘法测定两联药方的部分抑菌浓度(FIC)。结果显示,阿米卡星、多粘菌素B和环丙沙星对米尔伊丽莎白菌和肺炎克雷伯菌的抑菌效果较好,最小抑菌浓度(MIC)分别为2.5μg/mL、5-10μg/mL和10μg/mL;五倍子、金诃子和金银花抑菌效果较好,MIC分别为31.25 mg/mL、125 mg/mL和500 mg/mL;这三种抑菌效果较好的中草药之间两两联用未发生拮抗作用。中西两联药方中,五倍子和多粘菌素B对米尔伊丽莎白菌的抑菌效果较好,FIC为1,表现为相加作用,可作为黑斑蛙“歪头病”抑菌药物的参考配伍组合。To investigate the in vitro antibacterial effects of commonly used antibiotics and traditional Chinese medicines on the pathogenic bacteria caused torticollis in black-spotted frogs,the sensitivity of Elizabethkingia miricola and Klebsiella pneumoniae to 9 commonly used antibiotics and 6 traditional Chinese medicines was determined using the disk diffusion method and the double dilution method.The fractional inhibitory concentration(FIC)of two-drug combinations was further measured using the checkerboard method.The results showed that Amikacin,Polymyxin B,and Ciprofloxacin had better antibacterial effects against both pathogens,with minimum inhibitory concentrations(MIC)of 2.5μg/mL,5-10μg/mL,and 10μg/mL,respectively;Rhus chinensis Mill.,Terminalia chebula Retz.,and Lonicerae japonicae had good antibacterial effects,with MIC values of 31.25 mg/mL,125 mg/mL,and 500 mg/mL,respectively.These three traditional Chinese medicines did not show antagonism when used in combination.Among the combinations between Chinese medicines and antibiotics,R.chinensis Mill.and Polymyxin B had a good antibacterial effect on E.miricola,with an FIC of 1,indicating an additive effect.This combination could be served as a reference for treatment of torticollis in black-spotted frogs.

关 键 词：黑斑蛙 歪头病 米尔伊丽莎白菌 肺炎克雷伯菌 复方 抑菌 

分 类 号：S865.4[农业科学—野生动物驯养]