40种中草药及其复方对弗氏柠檬酸杆菌的体外抑菌作用  

In vitro bacteriostatic studies on Citrobacter freundii by 40 Chineseherbal medicines and their combinations

作　　者：朱能斌 陈毅凌 岳华梅 刘洪明 徐洪森 ZHU Nengbin;CHEN Yiling;YUE Huamei;LIU Hongming;XU Hongsen(School of Animal Science and Nutritional Engineering,Wuhan Polytechnic University,Wuhan 430023,China;Key Lab of Freshwater Biodiversity Conservation Ministry of Agriculture and Rural Affairs of China,Yangtze River Fisheries Research Institute,CAFS,Wuhan,430223,China)

机构地区：[1]武汉轻工大学动物科学与营养工程学院,武汉430023 [2]农业农村部淡水生物多样性保护重点试验室长江水产研究所,武汉430223

出　　处：《武汉轻工大学学报》2024年第5期18-24,共7页Journal of Wuhan Polytechnic University

基　　金：湖北省自然科学基因项目(编号:2021CFB265);武汉轻工大学大学生创新创业训练计划项目(编号:2107020007).

摘　　要：为有效防治因弗氏柠檬酸杆菌(Citrobacter freundii)引发的水产疾病,测定了40种单方中草药及其复方中草药对克氏原螯虾(Procambarus clarkii)源弗氏柠檬酸杆菌的体外抑菌作用。运用平板打孔法测定40种单方中草药的抑菌圈大小,然后测定4种抑菌效果较好的中草药对弗氏柠檬酸杆菌的最小抑菌浓度和最小杀菌浓度,并对这4种中草药进行复方作用评价。实验结果表明,12种中草药具有抑菌作用,其中乌梅的(Fructus mume)抑菌圈直径为23.33±0.33 mm,最小抑菌浓度为31.25 mg/mL,抑菌等级为极度敏感。五倍子的抑菌圈直径为18.00±4.00 mm,最小抑菌浓度为62.5 mg/mL,为高度敏感。复方中草药中乌梅+五倍子组的抑菌效果最好,抑菌圈直径大小为16.50±0.41 mm,最小抑菌浓度和最小杀菌浓度分别为31.25 mg/mL和62.5 mg/mL。这一研究表明,单方中草药乌梅和复方中草药乌梅+五倍子对弗氏柠檬酸杆菌具有良好的抑制作用,可以用于水产养殖中克氏原螯虾弗氏柠檬酸杆菌病的防治。In order to find out the effective prevention and treatment of aquatic diseases caused by Citrobacter freundii,we investigated the in vitro bacteriostatic effects of forty individual Chinese herbal medicines and their combinations on C.freundii isolated form Procambarus clarkii.The dimensions of the inhibitory circle for the 40 individual Chinese herbal medicines were tested with plate perforation method,followed by testing both the minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of the 4 Chinese herbal medicines that exhibit superior inhibitory effects against C.freundii,and then the compounding effect of these four Chinese herbal medicines was evaluated.The results revealed that 12 Chinese herbal medicines possessed bacteriostatic properties,Fructus mume has the best antibacterial effect,with the inhibitory circle and the MIC were 23.33±0.33 mm and 31.25 mg/mL,respectively.Rhus chinensis exhibits the second susceptibility,with the inhibition circle and the MIC were 18.00±4.00 mm and 62.5 mg/mL,respectively.The best bacteriostatic effect was observed in the group of F.mume+R.chinensis combination,with the inhibition circle,MIC and MBC were 16.50 mm,31.25 mg/mL and 62.5 mg/mL,respectively.This study demonstrated that the single F.mume as well as the compound of F.mume+R.chinensis had good inhibitory effects on C.freundii and could be used for the prevention and control of C.freundii disease in P.clarkii aquaculture.

关 键 词：中草药 弗氏柠檬酸杆菌 体外抑菌效果 克氏原螯虾 

分 类 号：S947[农业科学—水产养殖]