狗牙根TCP转录因子基因家族鉴定与分析  

Genome-wide identification and analysis of TCP transcription factors in bermudagrass

作　　者：孙启雪 陈焯婷 陈荣荣 张兵 SUN Qixue;CHEN Zhuoting;CHEN Rongrong;ZHANG Bing(College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,Jiangsu,China;Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,Jiangsu,China)

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]江苏省中国科学院植物研究所,江苏南京210014

出　　处：《草业科学》2024年第10期2330-2344,共15页Pratacultural Science

基　　金：国家自然科学基金面上项目“蛋白激酶CdCIPK15A调控狗牙根株型的分子机制”(32072613)。

摘　　要：TCP转录因子是植物特有的一类转录因子,在植物生长发育过程发挥着关键作用。狗牙根(Cynodon dactylon)是被广泛应用的、具有重要经济价值的暖季型草坪草,具有发达的根状茎和匍匐茎。本研究从狗牙根基因组鉴定了65个分属于PCF、CIN、CYC/TB1亚族的TCP转录因子。染色体定位分析表明,65个TCP基因不均衡地分布于狗牙根36条染色体上;共线性分析表明,串联复制机制作用于TCP基因。研究结果表明,3个亚家族族内的TCP转录因子基因成员的基因结构、蛋白结构域均高度保守。亚细胞定位预测结果显示,TCP转录因子能够定位于细胞核、叶绿体和线粒体。启动子元件分析发现,TCP基因启动子区存在数量不等的激素应答顺式作用元件。转录组学数据显示,TCP基因具有不同的组织表达模式,该现象在匍匐茎和根状茎尤为明显。本研究为进一步探究TCP转录因子在狗牙根茎特化和生长发育过程中的作用奠定了基础。TCP transcription factors are a class of plant-specific transcription factors that play a key role in the regulation of plant growth and development.Bermudagrass(Cynodon dactylon)is a widely used and economically important warm-season turfgrass with well-developed rhizomes and stolons.In this study,65 TCP transcription factor genes belonging to three subfamilies,PCF,CIN and CYC/TB1,were successfully identified from the bermudagrass genome.Chromosomal localization analysis showed that the 65 TCP genes were unevenly distributed on 36 chromosomes of bermudagrass,and collinear analysis indicated that tandem replication mechanisms acted on these TCP genes.Analysis demonstrated that gene structures and protein structural domains of TCP transcription factors of the three subfamilies were highly conserved.Subcellular localization predictions showed that TCP transcription factors are able to localize in the nucleus,chloroplasts and mitochondria.Analysis of promoter elements revealed a variable number of hormone response cis-acting elements in the promoter regions of different TCP transcription factor genes.Transcriptomic data showed that TCP genes have different patterns of tissue expression,which were particularly evident in stolons and rhizomes.This study provides a basis for further exploration of the role of TCP transcription factors in stem specialization and growth and development of bermudagrass.

关 键 词：狗牙根 TCP转录因子 系统进化 共线性分析 生长发育 茎特化 基因表达谱 

分 类 号：S688.4[农业科学—观赏园艺]