杏鲍菇生物法絮凝斜生栅藻的工艺优化和絮凝机制  

Process Optimization and Flocculation Mechanism on Biological Flocculation of Scendesmus obliquus by Pleurotus eryngii

作　　者：孙利芹[1] 吉松松 王祥莹 李岩 SUN Liqin;JI Songsong;WANG Xiangying;LI Yan(School of Life Sciences,Yantai University,Yantai 264005,China)

机构地区：[1]烟台大学生命科学学院,山东烟台264005

出　　处：《烟台大学学报（自然科学与工程版）》2024年第4期380-387,共8页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：山东省自然科学基金资助项目(ZR2020MC043);山东省现代农业产业技术体系项目(SDAIT-26)。

摘　　要：为更好地探索生物絮凝法采收微藻细胞的可行性,考察了杏鲍菇菌丝球絮凝斜生栅藻的效果,在单因素和正交试验设计的基础上,优化了絮凝工艺,采用Zeta电位分析法、荧光分析和傅里叶红外光谱分析法从静电作用、胞外聚合物等角度探讨了真菌生物絮凝微藻的机制。实验结果表明,最佳的工艺条件为:pH=3、絮凝温度26℃、转速180 r/min、藻液初始吸光度A 0=0.6,此条件下絮凝率达到85.67%,且对絮凝效果的影响为藻液初始吸光度>转速=温度>时间。杏鲍菇菌丝球絮凝斜生栅藻以化学吸附为主,静电作用、蛋白质和腐殖酸等胞外聚合物、细胞表面官能团均影响絮凝效果。本研究将为利用真菌绿色安全地絮凝微藻、提高微藻采收率提供重要参考。To better explore the biological flocculation effect on microalgae,the flocculation of P.eryngii mycelium pellets on S.obliquus cells is investigated.The flocculation process is optimized based on single-factor and orthogonal experimental designs,then Zeta potentiometry,fluorometric analysis and fourier infrared spectroscopy are selected to disclose the mechanisms of fungal bioflocculation on microalgae from the perspectives of electrostatic interaction,extracellular polymers.The experimental results show that the optimum flocculation process are:pH=3,flocculation temperature 26℃,rotational speed 180 r/min,and initial microalgae absorbance A 0=0.6.The flocculation rate reaches 85.67%under the above conditions,and the impact sequence is initial microalgae absorbance>rotational speed=temperature>time.S.obliquus is flocculated by P.eryngii mycelium pellets mainly through chemisorption,and other factors including electrostatic interaction between the fungal and microalgal cells,extracellular polymers such as proteins and humic acids,functional groups of cell surface also perform important function.This study will provide important references for microalgae flocculation by fungi in a green safe way and improve the harvesting rate of microalgae.

关 键 词：斜生栅藻 杏鲍菇 生物絮凝 工艺优化 絮凝机制 

分 类 号：Q81[生物学—生物工程]