藏贝母缓解小鼠非酒精性脂肪肝纤维化作用的研究  

Mitigating effect of Tibetan Fritillaria cirrhosa D.Don on non-alcoholic fatty liver fibrosis in mice

作　　者：许晓光 梁家旭 王晓岩[3,4] 苗春伊 白马次仁 陈鹏 蒋晓莉 寇芳芳 文姝 XU Xiaoguang;LIANG Jiaxu;WANG Xiaoyan;MIAO Chunyi;BAIMA Ciren;CHEN Peng;JIANG Xiaoli;KOU Fangfang;WEN Shu(Tibetan Institute of Plateau Medicine,Tibet Autonomous Region People's Hospital,Lhasa,Tibet 850010,China;不详)

机构地区：[1]西藏自治区人民医院西藏高原医学研究所,西藏拉萨850010 [2]大连医科大学基础医学院 [3]大连医科大学附属二院体检中心 [4]那曲市人民医院高原医学研究院

出　　处：《中国微生态学杂志》2024年第9期1024-1030,共7页Chinese Journal of Microecology

基　　金：那曲地区特色植物提取开发及产业化示范研究(qyxtzx-nq2022-03)。

摘　　要：目的探究藏贝母对非酒精性脂肪肝纤维化进程的阻止作用。方法将30只6周龄雄性C57BL/6小鼠随机分为对照组(N组)、模型组(M组)和藏贝母治疗组(H组),每组10只。通过喂食高脂饮食和腹腔注射四氯化碳建立非酒精性脂肪肝纤维化模型,灌胃藏贝母悬浊液0.2 mL/只(每只鼠每天100 mg/kg)。通过HE染色、油红染色、MASSON染色等检测肝脏脂滴聚集、纤维化状况和肠屏障的恢复情况。通过酶联免疫吸附法和微板法检测小鼠的代谢和炎症指标,包括空腹血清葡萄糖、总胆固醇、肿瘤坏死因子α等。结果与M组相比,H组显著降低了肝脏的NAS评分(t=5.667,P=0.001),显著降低了肝脏的脂滴聚集,显著降低了小鼠的AST(t=9.338,P=0.001)和ALT(t=213.6,P<0.001)水平。与M组相比,H组显著降低了肝脏的纤维比例(t=2.693,P=0.036)并且显著降低了肝脏α-SMA(t=6.295,P=0.002)和TIMP-1(t=4.494,P=0.004)的表达,小鼠胰岛素抵抗呈降低趋势,血清甘油三酯(t=4.253,P=0.013)水平显著下降。与M组相比,H组显著修复了模型小鼠的十二指肠和结肠基底层结构,并显著增加分泌型粘蛋白(t=2.989,P=0.024)的产生,同时H组显著抑制了模型小鼠血清LPS(t=6.690,P=0.003)的含量增加。结论藏贝母能显著阻止非酒精性脂肪肝纤维化进程,同时改善糖脂代谢,修复肠道屏障。Objective To explore the blocking effect of Tibetan Fritillaria cirrhosa D.Don on the fibrotic process of nonalcoholic fatty liver(NAFL)and its mechanism.Methods Thirty 6-week-old male C57BL/6 mice were randomly divided into control group(Group N),model group(Group M)and a Tibetan Fritillaria cirrhosa D.Don treatment group(Group H).A model of NAFL fibrosis was constructed by feeding a high-fat diet and intraperitoneal injection of carbon tetrachloride,with the intervention of gavage of Tibetan Fritillaria cirrhosa D.Don suspension at 0.2 mL/pupil(100 mg/kg/day for each mouse).Liver lipid droplet aggregation,fibrosis status and recovery of intestinal barrier were detected by using HE staining,oil red staining and MASSON staining.Metabolic and inflammatory indexes in mice were detected with enzyme-linked immunosorbent assay and microplate assay,including fasting serum glucose,total cholesterol and tumor necrosis factorα.Results Compared with group M,the NAS score of liver(t=5.667,P=0.001)reduced,lipid droplet aggregation in liver and levels of AST(t=9.338,P=0.001)and ALT(t=213.6,P<0.001)markedly reduced in group H.Compared with group M,group H significantly reduced the proportion of fibers in the liver(t=2.693,P=0.036)and notably decreased the expression of hepaticα-SMA(t=6.295,P=0.002)and TIMP-1(t=4.494,P=0.004),and mice showed a tendency of decreased insulin resistance,and the level of serum triglyceride(t=4.253,P=0.013)significantly decreased.Compared with group M,the structures of duodenal and colonic basal lamina of model mice in group H were prominently repaired,the production of secreted mucin(t=2.989,P=0.024)observably increased,while the increase of serum LPS(t=6.690,P=0.003)was significantly inhibited.Conclusion Tibetan Fritillaria cirrhosa D.Don can significantly inhibit the process of NAFL fibrosis,while improve glucose-lipid metabolism and repair intestinal barrier.

关 键 词：肝纤维化 藏贝母 糖脂代谢 

分 类 号：R575.1[医药卫生—消化系统]