双歧杆菌BB-12后生元对口腔变形链球菌的抑制作用  

Inhibitory effect of postbiotic Bifidobacterium BB-12 on oral Streptococci

作　　者：谷巍 兰玲莉 杨光[1] GU Wei;LAN Lingli;YANG Guang(Department of Stomatology,Affiliated Hospital of Chengde Medical College,Chengde,Hebei 067000,China;不详)

机构地区：[1]承德医学院附属医院口腔科,河北承德067000

出　　处：《中国微生态学杂志》2024年第9期1031-1034,1040,共5页Chinese Journal of Microecology

基　　金：2020年承德市科学技术研究与发展计划项目(202006A161)。

摘　　要：目的探讨双歧杆菌BB-12后生元对口腔变形链球菌的抑制作用,为预防和治疗口腔疾病提供新思路。方法本研究将双歧杆菌BB-12后生元与口腔变形链球菌共培养,按不同培养模式分为Control组(变形链球菌阳性对照组)、A组(变形链球菌+双歧杆菌组)、B组(变形链球菌+灭活双歧杆菌组)和C组(变形链球菌+发酵双歧杆菌组)。收集培养24 h后的各组菌体,Trizol法提取菌体总RNA,荧光定量PCR法检测葡糖基转移酶gtfB、gtfC基因、果糖基转移酶ftf基因、葡聚糖结合蛋白gbpB、gbpC基因和组氨酸激酶ciaH、comD基因的表达量。结果与Control组比较,其余3组的口腔变形链球菌gtfB、gtfC、ftf、gbpB、gbpC、ciaH、comD基因表达量均显著降低(均P<0.05);实验组组间比较,差异无统计学意义(P>0.05)。结论双歧杆菌BB-12后生元可能通过下调变形链球菌中与生物膜形成等相关基因的表达,抑制口腔变形链球在口腔黏膜上形成生物膜,防止口腔疾病的发生。Objective To explore the inhibitory effect of postbiotic Bifidobacterium BB-12 on oral Streptococci,and provide new ideas for the prevention and treatment of oral diseases.Methods The postbiotic Bifidobacterium BB-12 was co-cultured with oral Streptococci,and divided into four groups according to the culture modes:Control group(Streptococci positive control),group A(Streptococci+Bifidobacterium),group B(Streptococci+inactivated Bifidobacterium)and group C(Streptococci+fermented Bifidobacterium).The organisms in each group were collected 24 hours after cultivation,and total RNA of the organisms were extracted using Trizol method,and fluorescence quantitative PCR was used to detect the expressions of glucosyltransferase gtfB and gtfC genes,fructosyltransferase ftf gene,glucan-binding protein gbpB and gbpC genes,and histidine kinase ciaH and comD genes.Results Compared with the Control group,the expressions of oral Streptococci gtfB,gtfC,ftf,gbpB,gbpC,ciaH,and comD genes significantly reduced in the three observation groups(all P<0.05);there were no significant differences among the three observation groups(P>0.05).Conclusion The postbiotic Bifidobacterium BB-12 may inhibit oral Streptococci by down-regulating the expression of genes related to biofilm formation in Streptococci,thus preventing its biofilm formation on oral mucosa and preventing the occurrence of oral diseases.

关 键 词：双歧杆菌BB-12 后生元 口腔变形链球菌 抑制作用 

分 类 号：R780.2[医药卫生—口腔医学]