金钗石斛多糖调节Plin5介导心肌细胞脂肪酸β氧化改善糖尿病大鼠心肌损伤  

Dendrobium Nobile Polysaccharide Improves Myocardial Injury in Diabetic Rats by Regulating Plin5 Mediated Fatty AcidβOxidation of Cardiomyocytes

作　　者：李继舟 李宏伟 汤胜宇 姜泽军 郭萌萌 李雅甜 杨红玲 LI Jizhou;LI Hongwei;TANG Shengyu;JIANG Zejun;GUO Mengmeng;LI Yatian;YANG Hongling(School of Clinical Medicine University,Dali University,Dali Yunnan 671003;Dept.of Cardiovascular Medicine,The 3rd People’s Hospital of Yunnan Province,Kunming Yunnan 650011,China)

机构地区：[1]大理大学临床医学院,云南大理671003 [2]云南省第三人民医院心血管内科,云南昆明650011

出　　处：《昆明医科大学学报》2024年第10期8-16,共9页Journal of Kunming Medical University

基　　金：云南省教育厅科学研究基金资助项目(2023J0924,2024Y934)。

摘　　要：目的从Plin5调节心肌细胞脂肪酸β氧化信号通路探究金钗石斛多糖干预糖尿病大鼠心肌损伤的作用机制。方法清洁级雄性SD大鼠共72只,将其分为正常组(n=10)以及造模组(n=62),正常组给予普通饲料喂养,造模组给予高脂高糖饮食2月+一次性STZ注射构建糖尿病心肌损伤动物模型,构建模型成功后,将造模组SD大鼠分为5组,分别为模型组、单硝酸异山梨酯片组、金钗石斛多糖低剂量组、中剂量组和高剂量组(n=10),药物治疗4周后,处死大鼠,收集心脏,检测心肌组织病理学(HE和Masson染色)、心脂质含量(TG、TC、HDL-C和LDL-C)、心肌炎性因子(IL-1α、IL-1β、IL-6和TNF-α)和抗氧化指标(SOD、GSH、MDA和ROS),收集血液检测心功能、胰岛素和血糖水平,采用Western blot检测心肌组织中Plin5、SCD1、CPT1A、ACOX1和PPARα蛋白表达特点。结果组织病理学HE和Masson染色显示正常组大鼠的心肌纤维整齐排列,模型组大鼠出现心肌纤维断裂,与模型组相比,金钗石斛多糖各治疗组可见不同程度减轻;相较于正常组,模型组血糖和胰岛素抵抗指数升高(P<0.05),胰岛素下降(P<0.05),相较于模型组,金钗石斛多糖3个组血糖和胰岛素抵抗指数降低(P<0.05),低剂量组、中剂量和硝酸异山梨酯片组胰岛素水平没有升高(P>0.05),金钗石斛多糖高剂量组胰岛素含量升高(P<0.05);相较于正常组,模型组TG、TC和LDL-C升高(P<0.05),HDL-C降低(P<0.05),相较于模型组,金钗石斛多糖治疗组以及阳性药物组TG、TC和LDL-C降低(P<0.05),HDL-C升高(P<0.05);相较于正常组,模型组AST、CK、CK-MB、LDH、a-HBDH以及CTnl升高(P<0.05),相较于模型组,金钗石斛多糖3个治疗组和阳性药物组上述6个指标降低(P<0.05);相较于正常组,模型组心肌IL-1α、IL-1β、IL-6和TNF-α升高(P<0.05),相较于模型组,金钗石斛多糖3个治疗组和阳性药物组上述炎性因子指标降低(P<0.05);相较于正常组,模型组SOD和GSH降�Objective To explore the mechanism of Dendrobium nobium polysaccharide on myocardial injury in diabetic rats by regulating the fatty acidβoxidation signaling pathway of cardiomyocytes from Plin5.Methods A total of 72 male SD rats of a clean grade were divided into a control group(n=10)and a modeling group(n=62).The control group was fed regular diet,while the modeling group was given a high-fat,high-sugar diet for two months,along with a single STZ injection to establish a diabetic myocardial injury animal model.After successfully creating the model,the modeling group of SD rats was further divided into five subgroups:the model group,the isosorbide mononitrate tablet group,the low-dose Dendrobium nobium polysaccharide group,the medium-dose group,and the high-dose group(n=10).After 4 weeks of drug treatment,rats were euthanized,and their hearts were collected to detect the myocardial hemopathology(using HE and Masson staining),lipid levels(TG,TC,HDL-C and LDL-C),inflammatory factors(IL-1α,IL-1β,IL-6 and TNF-α)and antioxidant indexes(SOD,GSH,MDA and ROS).Blood samples were taken to assess cardiac function,insulin and blood sugar levels,and Western blot was used to detect the expression characteristics of Plin5,SCD1,CPT1A,ACOX1 and PPARαproteins.Results Histopathology with HE and Masson staining showed that in the normal group,the myocardial fibers of rats were neatly arranged,whereas in the model group,there was fibrous rupture.Compared to the model group,different degrees of improvement were seen in all treatment groups with Dendrobium polysaccharides.Blood sugar and insulin resistance index increased in the model group compared to the normal group(P<0.05),with insulin levels decreasing(P<0.05).Compared to the model group,the three groups treated with Dendrobium polysaccharides showed reduced blood sugar and insulin resistance index(P<0.05).In the low-dose,mid-dose,and isosorbide dinitrate tablet groups,insulin levels did not increase(P>0.05),while the high-dose Dendrobium polysaccharides group exhibited an i

关 键 词：钗石斛多糖 Plin5 脂肪酸β氧化 糖尿病心肌损伤 

分 类 号：R575.45[医药卫生—消化系统]