基因编辑猪-猴异种组织器官移植围手术期动物护理  

Perioperative Animal Care for Xenotransplantation from Genetically Edited Pigs to Monkeys

作　　者：朱婵 张栋梁 赵德莉 石雪琴 千磊 张玄 金艳 段伟 戚若晨 刘超华 杨薛康 韩军涛 潘登科 ZHU Chan;ZHANG Dongliang;ZHAO Deli;SHI Xueqin;QIAN Lei;ZHANG Xuan;JIN Yan;DUAN Wei;QI Ruocheng;LIU Chaohua;YANG Xuekang;HAN Juntao;PAN Dengke(Department of Burns and Cutaneous Surgery,Burn Center of PLA,the First Affiliated Hospital,Air Force Medical University,Xi'an 710032,China;Laboratory Animal Centre,Air Force Medical University,Xi'an 710032,China;Department of Hepatobiliary Surgery,the First Affiliated Hospital,Air Force Medical University,Xi'an 710032,China;Department of Cardiac Surgery,the First Affiliated Hospital,Air Force Medical University,Xi'an 710032,China;Department of Orthopedics,the First Affiliated Hospital,Air Force Medical University,Xi'an 710032,China;Department of Urinary Surgery,the First Affiliated Hospital,Air Force Medical University,Xi'an 710032,China;Department of Plastic Surgery,the First Affiliated Hospital,Air Force Medical University,Xi'an 710032,China;Chengdu Clonorgan Biotechnology Co.,Ltd.,Chengdu 610095,China)

机构地区：[1]空军军医大学第一附属医院全军烧伤中心/烧伤与皮肤外科,西安710032 [2]空军军医大学实验动物中心,西安710032 [3]空军军医大学第一附属医院肝胆外科,西安710032 [4]空军军医大学第一附属医院心脏外科,西安710032 [5]空军军医大学第一附属医院骨科,西安710032 [6]空军军医大学第一附属医院泌尿外科,西安710032 [7]空军军医大学第一附属医院整形外科,西安710032 [8]成都中科奥格生物科技有限公司,成都610095

出　　处：《实验动物与比较医学》2024年第5期495-501,共7页Laboratory Animal and Comparative Medicine

基　　金：陕西省重点研发计划“TIGIT-Fc重组蛋白诱导异种移植免疫耐受的机制及应用”(2021SF-107);空军军医大学第一附属医院2024年度医务人员培养助推立项资助计划“多基因编辑猪皮用于深度烧伤创面的安全性和有效性研究”(XJZT24LZ06)。

摘　　要：目的探讨基因编辑猪-猴异种组织器官移植围手术期的动物护理,为提高猪-猴异种组织器官移植实验的成功率提供帮助。方法2022年10月—2023年10月,对行基因编辑猪-猴异种组织器官移植的7只受体恒河猴进行围手术期护理和伤口保护。根据体型及手术区域,为受体猴定制伤口防护服以保护伤口,同时做好围手术期精细化护理。其中,术前护理包括术前准备、术前用药等;术中护理包括生理指标监护和麻醉手术护理等;术后护理包括伤口保护、观察监测、营养护理等。结果7只恒河猴的异种组织器官移植手术均获得成功。通过伤口防护服的保护和精细化护理,所有恒河猴手术伤口均一期愈合,术后恢复良好。结论做好猪-猴异种组织器官移植围手术期的护理和伤口保护,不仅能促进伤口愈合,还能减轻手术给动物带来的疼痛和伤害,这对猪-猴异种组织器官移植的实验研究进展和提升动物福利均具有重要意义。Objective To discuss the perioperative care and wound protection of xenotransplantation from genetically edited pigs to monkeys,with the goal of improving the success rate of such experimental procedures.Methods From October 2022 to October 2023,perioperative care and wound protection were performed on 7 recipient rhesus monkeys undergoing xenotransplantation of genetically edited pig tissues and organs.Customized wound protective garments were designed based on monkeys'size and surgical area to protect the wounds,alongside meticulous perioperative care.This included preoperative preparation and medication,intraoperative monitoring of physiological indicators and anesthesia management,and postoperative care comprising wound protection,observation and monitoring,and nutritional support.Results All seven monkeys successfully underwent xenotransplantation.With the aid of protective garments and detailed care,all surgical wounds healed by first intention,and postoperative recovery was satisfactory.Conclusion Proper care and wound protection during xenotransplantation from genetically edited pigs to monkeys not only promote wound healing,but also alleviate pain and harm to animals.This has significant implications for advancing experimental research in pig-monkey xenotransplantation and enhancing animal welfare.

关 键 词：基因编辑猪 异种器官移植 恒河猴 护理 伤口保护装置 实验动物福利 

分 类 号：R-332[医药卫生] Q95-33[生物学—动物学]