Circ_0103552靶向miR-1200调控肺癌A549细胞增殖、迁移及侵袭  

Mechanism of circ_0103552 regulating lung cancer A549 cell proliferation,migration and invasion by targeting miR-1200

作　　者：周方[1] 王猛[1] 程世钊 王峥[1] 张思培 卢喜科 ZHOU Fang;WANG Meng;CHENG Shi-zhao;WANG Zheng;ZHANG Si-pei;LU Xi-ke(Dept of Thoracic Surgery,Chest Hospital,Tianjin University,Tianjin 300222,China;Dept of Pharmacy,Chest Hospital,Tianjin University,Tianjin 300222,China)

机构地区：[1]天津大学胸科医院胸外科,天津300222 [2]天津大学胸科医院药剂科,天津300222

出　　处：《中国药理学通报》2024年第11期2099-2104,共6页Chinese Pharmacological Bulletin

基　　金：新疆维吾尔自治区卫生健康青年医学科技人才专项科研项目(No WJWY-202023);天津市医学重点学科建设项目(No TJYXZDXK-018A)。

摘　　要：目的探讨circ_0103552对人肺癌A549细胞增殖、迁移及侵袭的影响及其可能作用机制。方法qRT-PCR法检测circ_0103552、miR-1200在肺癌组织、癌旁组织表达;A549细胞分为si-NC、si-circ_0103552、miR-NC、miR-1200、si-circ_0103552+anti-miR-NC、si-circ_0103552+anti-miR-1200组;检测细胞增殖、克隆形成、迁移和侵袭;采用双荧光素酶报告实验验证circ_0103552与miR-1200的靶向调控关系。Western blot检测E-cadherin、N-cadherin蛋白表达量。结果与癌旁组织比较,肺癌组织中circ_0103552的表达量升高(P<0.05),miR-1200的表达量降低(P<0.05);Circ_0103552下调或者过表达miR-1200削弱细胞活力、N-cadherin、克隆、侵袭和促进E-cadherin蛋白(P<0.05);circ_0103552可靶向miR-1200;下调miR-1200可逆转circ_0103552敲低对A549细胞增殖,迁移,侵袭的抑制作用以及对细胞凋亡的促进作用(P<0.05)。结论Circ_0103552通过靶向miR-1200促进肺癌细胞的进展。Aim To explore the effect of circ_0103552 on the proliferation,migration and invasion of lung cancer A549 cells and its possible mechanism.Methods qRT-PCR was used to detect circ_0103552 and miR-1200 expression in lung cancer tissues and adjacent tissues.A549 cells were grouped into si-NC,si-circ_0103552,miR-NC,miR-1200,si-circ_0103552+anti-miR-NC,si-circ_0103552+anti-miR-1200 groups.Cell proliferation,clone formation,migration and invasion were detected.The targeting relationship between circ_0103552 and miR-1200 was assessed using the dual luciferase reporter experiment.The expression of E-cadherin and N-cadherin protein was detected by Western blot.Results Compared with adjacent tissues,circ_0103552 expression in lung cancer tissue increased(P<0.05),while miR-1200 expression decreased(P<0.05).Circ_0103552 knockdown or miR-1200 overexpression reduced cell viability,N-cadherin,cloning,invasion,and promoted E-cadherin protein(P<0.05).Circ_0103552 could target miR-1200.Down-regulating miR-1200 could reverse the inhibitory effect of circ_0103552 knockdown on proliferation,migration and invasion,as well as the promotion effect on cell apoptosis(P<0.05).Conclusion Circ_0103552 could promote lung cancer cell progression by targeting miR-1200.

关 键 词：肺癌 circ_0103552 miR-1200 增殖 迁移 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]