The glutamate receptor gene GLR3.3:A bridge of calciummediated root development in poplar  

作　　者：Yi An Ya Geng Yu Liu Xiao Han Lichao Huang Wei Zeng Jin Zhang Mengzhu Lu 

机构地区：[1]State Key Laboratory of Subtropical Silviculture,Sino-Australia Plant Cell Wall Research Centre,College of Forestry and Biotechnology,Zhejiang A&F University,Hangzhou,Zhejiang 311300,China

出　　处：《Horticultural Plant Journal》2024年第6期1449-1462,共14页园艺学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.32371902,31901327);National Key Research and Development Program of China(Grant Nos.2019YFE0119100,2021YFD2200205);Overseas Expertise Introduction Project for Discipline Innovation(111 Project D18008);The researches foundation of Zhejiang A&F University(Grant No.2018FR013)。

摘　　要：Poplar is one of the fastest-growing temperate trees in the world and is widely used in ornamental horticulture for shade.The root is essential for tree growth and development and its utilization potential is huge.Calcium(Ca),as a signaling molecule,is involved in the regulation of plant root development.However,the detailed underlying regulatory mechanism is elusive.In this study,we analyzed the morphological and transcriptomic variations of 84K poplar(Populus alba×P.glandulosa)in response to different calcium concentrations and found that low Ca^(2+)(1 mmol·L^(-1))promoted lateral root development,while deficiency(0.1 mmol·L^(-1)Ca^(2+))inhibited lateral root development.Co-expression analysis showed that Ca^(2+)channel glutamate receptors(GLRs)were present in various modules with significance for root development.Two GLR paralogous genes,PagGLR3.3a and Pag GLR3.3b,were mainly expressed in roots and up-regulated under Ca^(2+)deficiency.The CRISPR/Cas9-mediated signal gene(crispr-PagGLR3.3a,PagGLR3.3b)and double gene(crispr-PagGLR3.3ab)mutants presented more and longer lateral roots.Anatomical analysis showed that crispr-PagGLR3.3ab plants had more xylem cells and promoted the development of secondary vascular tissues.Further transcriptomic analysis suggested that knockout of PagGLR3.3a and PagGLR3.3b led to the up-regulation of several genes related to protein phosphorylation,auxin efflux,lignin and hemicellulose biosynthesis as well as transcriptional regulation,which might contribute to lateral root growth.This study not only provides novel insight into how the Ca^(2+)channels mediated root growth and development in trees,but also provides a directive breeding of new poplar species for biofuel and bioenergy production.

关 键 词：Glutamate receptor CALCIUM Root development Lateral root POPLAR 

分 类 号：S792.11[农业科学—林木遗传育种]