诺丽果黄酮对高精料底物条件下绒山羊体外瘤胃发酵的影响  

Effects of Noni Fruit Flavonoids on in Vitro Rumen Fermentation of Cashmere Goats with High⁃Concentrate Substrate

作　　者：张清月 董姝慧 李胤豪 刘锦涛 张尚雄 于浩 赵艳丽[1] 郭晓宇[1] 郭咏梅[1] 史彬林[1] 闫素梅[1] ZHANG Qingyue;DONG Shuhui;LI Yinhao;LIU Jintao;ZHANG Shangxiong;YU Hao;ZHAO Yanli;GUO Xiaoyu;GUO Yongmei;SHI Binlin;YAN Sumei(Key Laboratory of Animal Nutrition and Feed Science at Universities of Inner Mongolia Autonomous Region,College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China;National Center of Pratacultural Technology Innovation(Under Preparation),Hohhot 010070,China)

机构地区：[1]内蒙古农业大学动物科学学院,内蒙古自治区高校动物营养与饲料科学重点实验室,呼和浩特010018 [2]国家草业技术创新中心(筹),呼和浩特010070

出　　处：《动物营养学报》2024年第10期6459-6467,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家草业技术创新中心(筹)重大创新平台建设专项(CCPTZX2023B06);内蒙古自治区高校基本业务费(BR22-13-13)。

摘　　要：本试验旨在从诺丽果多酚(NFP)和诺丽果黄酮(NFF)中筛选出具有调控高精料底物条件下绒山羊体外瘤胃发酵的诺丽果提取物,并确定其适宜添加剂量,为绒山羊绿色饲料添加剂的开发及诺丽果资源的合理利用提供理论支撑。本试验以阿尔巴斯白绒山羊为瘤胃液供体动物,分两部分进行体外瘤胃发酵试验。第1部分:以白藜芦醇(RSV)为阳性对照,采用单因素完全随机试验设计,设4个处理,分别以高精料饲粮(精料∶粗料=65∶35)以及在高精料饲粮中添加0.25%NFP、0.25%NFF、0.02%RSV为底物进行体外瘤胃发酵,每个处理6个重复。第2部分:采用单因素完全随机试验设计,设5个处理,分别以添加0、0.025%、0.050%、0.100%和0.250%NFF的高精料饲粮为底物进行体外瘤胃发酵,每个处理6个重复。第1部分结果表明:NFF促进瘤胃发酵的多项组合效应值(MFAEI)最高,且其在缓解发酵液pH下降、降低发酵液中乳酸和组胺(HIS)浓度以及提高氮利用效率方面的调控作用均优于NFP。因而,筛选出NFF为对绒山羊体外瘤胃发酵具有较好调控作用的诺丽果提取物进行后续试验。第2部分结果表明:高精料底物中添加0.100%NFF时促进瘤胃发酵的MFAEI最高,同时其可显著降低体外发酵液中异常代谢物HIS和脂多糖(LPS)的浓度(P<0.05),并显著缓解高精料底物条件下pH的下降(P<0.05)。综上可知,NFF具有较好的促进绒山羊体外瘤胃发酵的效果,其在高精料底物中的适宜添加剂量为0.100%。This experiment aimed to screen one extract of noni fruit from noni fruit polyphenols(NFP)and noni fruit flavonoids(NFF),which can regulate the in vitro rumen fermentation of cashmere goats under the condition of high-concentrate substrate,and to screen the appropriate amount of its additives to provide theoreti-cal basis for the development of green feed additives for cashmere goats and the rational utilization of noni fruit resources.The experiment was conducted in two parts using Albas white cashmere goats as rumen fluid donor animals.Part 1:resveratrol(RSV)was used as a positive control.A single-factor completely randomized trial design was used to divide the fermentation bottles into four treatments with high-concentrate diet(the ratio of concentrate to roughage=65∶35)and the high-concentrate diet supplemented with 0.25%NFP,0.25%NFF and 0.02%RSV as substrates,respectively,to conducted the in vitro rumen fermentation experiment.Each treatment was performed in six replicates.Part 2:a single-factor completely randomized trial design was used to divide the fermentation bottles into five treatments with the high-concentrate diet supplemented with 0,0.025%,0.050%,0.100%and 0.250%NFF as substrates,respectively,to conducted the in vitro rumen fer-mentation experiment.The results of the part 1 showed that NFF promoted rumen fermentation with the highest of multiple forages associative effect indicator(MFAEI),and its regulatory effect was better than that of NFP in alleviating pH decline,reducing concentrations of lactic acid and histamine(HIS),and improving nitrogen utilization efficiency in the fermentation broth.Therefore,NFF was screened as a noni fruit extract with better modulation of rumen fermentation in vitro in cashmere goats for subsequent experiment.The results of the part 2 showed that the highest MEAEI to promote rumen fermentation was observed when 0.100%NFE was added to the high-concentrate substrate,while it significantly reduced the concentrations of abnormal metabolites HIS and lipopolysaccharide(

关 键 词：诺丽果 黄酮 多酚 高精料 绒山羊 体外瘤胃发酵 

分 类 号：S816[农业科学—饲料科学]