Pathogenicity and transcriptomic profiling reveals immunology molecular hallmarks after CA10 virus infection  

作　　者：Wanjun Peng Jing Wu Binbin Zhao Lihong Zhang Xin Chen Xiaohui Wei Na Rong Yunlin Han Jiangning Liu 

机构地区：[1]NHC Key Laboratory of Human Disease Comparative Medicine,Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases,Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences and Comparative Medicine Center,Peking Union Medical College,Beijing,China

出　　处：《Animal Models and Experimental Medicine》2024年第5期717-731,共15页动物模型与实验医学（英文）

基　　金：supported by the National Key Research and Development Program of China(Grant No.2022YFC2303404);the CAMS Innovation Fund for Medical Sciences(CIFMS)grant(2021-1-I2 M-035,2022-I2M-1-020)。

摘　　要：Background:Hand,foot and mouth disease(HFMD)is a common infectious disease caused by viral infection by a variety of enteroviruses,with coxsackievirus A 10(CA10)having become more prevalent in recent years.Methods:In this study,models of CA10 infection were established in 7-day-old Institute of Cancer Research(ICR)mice by intraperitoneal injection to analyze the pathogenicity of the virus.RNA sequencing analysis was used to screen the differentially expressed genes(DEGs)after CA10 infection.Coxsackievirus A 16(CA16)and enterovirus 71(EV71)infections were also compared with CA10.Results:After CA10 virus infection,the mice showed paralysis of the hind limbs at 3 days post infection and weight loss at 5 days post infection.We observed viral replication in various tissues and severe inflammatory cell infiltration in skeletal muscle.The RNA-sequencing analysis showed that the DEGs in blood,muscle,thymus and spleen showed heterogeneity after CA10 infection and the most upregulated DEGs in muscle were enriched in immune-related pathways.Compared with CA16 and EV71 infection,CA10 may have an inhibitory effect on T helper(Th)cell differentiation and cell growth.Additionally,the common DEGs in the three viruses were most enriched in the immune system response,including the Toll-l ike receptor pathway and the nucleotide-binding and oligomerization domain(NOD)-l ike pathway.Conclusions:Our findings revealed a group of genes that coordinate in response to CA10 infection,which increases our understanding of the pathological mechanism of HFMD.

关 键 词：CA10 HFMD RNA-Seq 

分 类 号：R392[医药卫生—免疫学]