荔枝蒂蛀虫不同地理种群遗传多样性初步研究  

Preliminary Study on Genetic Diversity of Different Geographical Populations of Conopomorpha sinensis Bradley

作　　者：郭义 李鹏燕[1] 李敦松[1] GUO Yi;LI Pengyan;LI Dunsong(Plant Protection Research Institute,Guangdong Academy of Agricultural Sciences/Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China,Ministry of Agriculture and Rural Aff airs/Guangdong Key Laboratory of High Technology for Plant Protection,Guangzhou 510640,China)

机构地区：[1]广东省农业科学院植物保护研究所/农业农村部华南果蔬绿色防控重点实验室/广东省植物保护新技术重点实验室,广东广州510640

出　　处：《广东农业科学》2024年第9期119-128,共10页Guangdong Agricultural Sciences

基　　金：国家荔枝龙眼产业技术体系专项(CARS-32);广州市科技计划项目(2024E04J1256)。

摘　　要：【目的】明确荔枝蒂蛀虫(Conopomorpha sinensis Bradley)不同地理种群的遗传结构特征、遗传多样性水平及分子变异情况,为其起源和演化研究提供科学证据,为不同区域的荔枝蒂蛀虫防治提供参考信息。【方法】选用我国南方5个产区的荔枝蒂蛀虫为材料,利用GBS(Genotyping-by-sequencing)技术对样本进行基因分型,再利用SAMTOOLS软件鉴定单核苷酸多态性(SNP)位点,根据SNP基因分型结果构建系统进化树,并进行主成分分析。【结果】利用GBS技术对5个地区荔枝蒂蛀虫进行测序,共获得高质量有效数据27.39 Gb(平均每个样本5.48 Gb)、高质量SNP位点11767个。系统进化树分析表明,四川泸州种群单独聚为一支,与其他4个种群亲缘关系较远。主成分分析结果显示,5个地区荔枝蒂蛀虫可划分为2个类群,四川泸州种群单独为一簇,明显区分于其他4个种群。主成分分析和系统进化树分析均表明不同地理来源的荔枝蒂蛀虫在遗传水平上存在明显差异。种群遗传多样性分析结果表明,5个种群观测杂合度(Ho)为0.2099~0.2531、期望杂合度(He)为0.1937~0.2203、核苷酸多样性(π)为0.2495~0.2748,表明5个荔枝蒂蛀虫种群的遗传多样性水平较低。AMOVA分析结果表明,荔枝蒂蛀虫种群间的遗传变异(0.87%)小于种群内(99.13%),表明变异主要来源于种群内而不是种群间。【结论】不同地区的荔枝蒂蛀虫种群遗传多样性水平较低,其中,四川泸州种群遗传分化程度较高,与其他4个种群亲缘关系较远。【Objective】The study was conducted to clarify the genetic structure characteristics,genetic diversity level and molecular variation of different geographical populations of Conopomorpha sinensis Bradely,with an aim to provide scientific evidence for the study of its origin and evolution and provide reference information for the control of this pest in different regions.【Method】Using the C.sinensis from 5 production areas in southern China as materials,using GBS(Genotyping-by-sequencing)technology was used for samples genotyping,then SNP locus was identified with SAMTOOLS software,and phylogenetic tree was constructed according to SNP genotyping results.Finally,principal component analysis was performed.【Result】Sequencing of C.sinensis from 5 regions was conducted by GBS technology.A total of 27.39 Gb of highquality effective data were obtained,with an average of 5.48 Gb per sample.A total of 11767 high-quality single nucleotide polymorphism(SNPs)loci were obtained.Phylogenetic tree analysis showed that the genetic relationship of Luzhou population was far from that of other populations.The results of principal component analysis showed that C.sinensis was divided into 2 groups in 5 regions,and the Luzhou population was significantly different from the other 4 populations.Both principal component analysis and phylogenetic tree analysis revealed that there were obvious differences in genetic level among C.sinensis from different geographical origins.The results of genetic diversity analysis showed that the observed heterozygosity was 0.2099-0.2531,the expected heterozygosity was 0.1937-0.2203,πwas 0.2495-0.2748,indicating that the genetic diversity level of C.sinensis population was low.The results of AMOVA analysis showed that the genetic variation between populations(0.87%)was smaller than that within populations(99.13%),indicating that the variation mainly came from within populations rather than between populations.【Conclusion】The genetic diversity level of C.sinensis in different geographical

关 键 词：荔枝蒂蛀虫 地理种群 遗传多样性 遗传分化 简化基因组测序 系统进化树 

分 类 号：S476.2[农业科学—农业昆虫与害虫防治]