UDP-糖基转移酶产生菌发酵牡丹皮的研究  

Study on fermentation of Moutan Bark by UDP-glycosyltransferase Producing Bacteria

作　　者：陈宝怡 李静 黄俊达 邓毛程 吴林杰 潘英 林英杰 郭沛鑫 豆志勇 周浩 林钰华 彭珍玲 刘浩畅 CHEN Baoyi;LI Jing;HUANG Junda;DENG Maocheng;WU Lingjie;PAN Ying;LIN Yingjie;GUO Peixin;DOUZhiyong;ZHOU Hao;LIN Yuhua;PENG Zhenhua;LIU Haochang(Guangdong Industry Polytechnic University,Guangzhou 510300,China)

机构地区：[1]广东轻工职业技术大学,广东广州510300

出　　处：《广东轻工职业技术大学学报》2024年第5期12-18,共7页Journal of Guangdong Industry Polytechnic university

基　　金：2024年广东轻工职业技术大学大学生挑战杯课外科技活动项目;广东省教育厅特色创新项目(2019GKTSCX011、2019GKTSCX012);广东轻工职业技术学院自然科学项目(KJ2020-020)。

摘　　要：为探索提高牡丹皮芍药苷提取水平的新途径,开展了UDP-糖基转移酶产生菌筛选及其对牡丹皮发酵的研究。从酱醅中筛选获得一株UDP-糖基转移酶产生菌GT16,经分子学鉴定为德巴利酵母(Debaryomyces prosopidis)。通过摇瓶发酵实验,确定了该菌种发酵的最佳温度和最佳初始pH分别为28℃和6.5。表面活性剂Triton X-100促进牡丹皮前体发酵转化为芍药苷效果明显,其最佳添加量为0.04%,最佳的发酵时间为72 h。研究表明,GT16菌种对牡丹皮的发酵转化作用明显,发酵样品的芍药苷浓度是未发酵对照样品的123.6%。Triton X-100对该菌种发酵的促进作用明显,添加Triton X-100的发酵样品的芍药苷浓度是无添加对照样品的115.9%。In order to explore a new approach to improve the extraction level of paeoniflorin from peony bark,the screening of UDP-glycosyltransferase producing bacteria and its effect on the fermentation of peony bark were carried out.An UDP-glycosyltransferase producing strain GT16 was isolated from fermented paste and identified as Debaryomyces prosopidis.The optimal fermentation temperature and initial pH of the strain GT16 were 28℃ and 6.5,respectively.Furthermore,the optimal addition amount of Triton X-100 to promote the fermentation of peony bark precursor into paeonidin was 0.04%,and the optimal fermentation time was 72 h.The fermentation and transformation effect of the strain GT16 on peony bark was significant.The concentration of paeoniflorin in the fermented sample was 123.6% of that in the unfermented control sample.Triton X-100 obviously promoted the fermentation of the strain GT16,and the concentration of paeoniflorin in the fermented sample added with Triton X-100 was 115.9% of that in the control sample without addition.

关 键 词：UDP-糖基转移酶 酵母 牡丹皮 芍药苷 

分 类 号：Q55[生物学—生物化学]