基于AFLP遗传多样性和功能成分含量的葛资源特性研究  

Characteristics of Pueraria montana Resources Based on AELP Genetic Diversity and Functional Component Content

作　　者：彭斯文[1] 龙世平[1] 朱校奇[1] 唐汉军[2] 谢进[1] 徐瑞[1] 黄艳宁[1] 曹亮[1] Peng Siwen;Long Shiping;Zhu Xiaoqi;Tang Hanjun;Xie Jin;Xu Rui;Huang Yanning;Cao Liang(Institute of Agriculture Environment and Agroecology,Hunan Academy of Agriculture Sciences,Changsha 410125,China;Institute of Agriculture Products Processing,Hunan Academy of Agriculture Sciences,Changsha 410125,China)

机构地区：[1]湖南省农业科学院湖南省农业环境生态研究所,湖南长沙410125 [2]湖南省农业科学院湖南省农产品加工研究所,湖南长沙410125

出　　处：《中国野生植物资源》2024年第10期9-16,共8页Chinese Wild Plant Resources

基　　金：国家现代农业产业技术体系专项资金(CARS-21)。

摘　　要：目的:研究葛资源的遗传多样性和品质特征。方法:提取葛幼嫩叶片基因组DNA,使用扩增片段长度多态性(AFLP)分子标记技术对各样品进行扩增,并应用GeneMapper分析软件对AFLP扩增结果进行分析;采用高效液相色谱(HPLC)对葛根中异黄酮类化合物葛根素、大豆苷、大豆甙元等主要成分进行含量测定,并利用SPSS软件进行成分含量的聚类分析。结果:5对AFLP荧光引物扩增获得1113条多态性条带,多态性比率达到100%。非加权组平均法(UPGMA)聚类分析结果显示,20号样品(湖南茶陵野葛)与其它葛资源差异较大,在相关系数为0.35时与其它样品分开,剩余样品在0.48的相关系数处分为两大类群,其中一类主要为野葛(云南、重庆),但有17号(湖南武冈粉葛)、18号样品(湖南江背粉葛)与该类聚集为一群,其遗传背景与野葛相似;而另一个类群主要为粉葛(广东、广西、湖南)和湖南的野葛资源,湖南野葛资源与云南等地区野葛差异较大。成分含量聚类分析结果表明所有样品可分属野葛与粉葛2个群,其中野葛的成分含量比粉葛高,但17号样品(湖南武冈粉葛)聚集在野葛群。结论:综合分析发现AFLP分子标记技术能揭示葛的遗传多样性,野葛及粉葛在成分含量方面存在明显差异,其中17号样品(湖南武冈粉葛)成分含量与野葛相近,为优异的粉葛资源。Objective:To investigate the genetic diversity and quality of Pueraria montana.Methods:The genomic DNA was extracted from young leaves of P.montana and AFLP molecular marker technology was used to amplify DNA samples.The AFLP amplification results were analyzed by GeneMapper software.High performance liquid chromatography(HPLC)was used to determine the content of isoflavonoids such as puerarin,daidzin,and daidzein in P.montana,and cluster analysis of component content was conducted using SPSS software.Results:Five pairs of AFLP fluorescent primers produced 1113 polymorphic bands,with a polymorphism rate of 100%.Unweighted pair-group method with arithmetic means(UPGMA)cluster analysis indicated that sample 20(P.montana var.lobata from Chaling,Hunan)showed significant differences from other resources,separating from the other samples at a correlation coefficient of 0.35.The remaining samples were divided into two major clusters at a correlation coefficient of 0.48.One cluster mainly comprised P.montana var.lobata(from Yunnan and Chongqing),but samples 17(P.montana var.thomsonii from Wugang,Hunan)and 18(P.montana var.thomsonii from Jiangbei,Hunan)clustered with this group,indicating similar genetic backgrounds to P.montana var.lobata.The other cluster primarily consisted of P.montana var.thomsonii(from Guangdong,Guangxi,and Hunan)and P.montana var.lobata in Hunan,with Hunan P.montana var.lobata resources showing significant differences from P.montana var.lobata in Yunnan and other regions.The content analysis results indicated that P.montana var.lobata and P.montana var.thomsonii belonged to two distinct groups,with P.montana var.lobata having higher component content than P.montana var.thomsonii.However,sample 17(P.montana var.thomsonii from Wugang,Hunan)clustered with P.montana var.lobata group,with high content of puerarin and daidzin.Conclusion:The genetic diversity of P.montana was revealed by AFLP molecular marker technology.There were significant differences in component content between P.montana var.lobata a

关 键 词：葛 粉葛 遗传多样性 AFLP 葛根素 

分 类 号：S602[农业科学—园艺学]