2型糖尿病合并乙型肝炎肝硬化相关肝细胞癌患者血清TNF-α、HSP70、sPD-1的水平及意义  

Serum levels of TNF-a,HSP70 and sPD-1 in hepatocellular carcinoma with hepatitis B cirrhosis and type 2 diabetes mellitus and their significance

作　　者：李矫捷 孙丽 张红红 LI Jiaojie;SUN Li;ZHANG Honghong(Infection Department of 148 Hospital in Zibo City,Zibo,Shandong,China,255300;Nursing Department of Infectious Diseases,People's Hospital of Linqing City,Liaocheng,Shandong,China,252600;Pathology Department of Qingdao Binhai University Affiliated Hospital,Qingdao,Shandong,China,266500)

机构地区：[1]淄博一四八医院感染科,山东淄博255300 [2]临清市人民医院感染科护理,山东聊城252600 [3]青岛滨海学院附属医院病理科,山东青岛266500

出　　处：《分子诊断与治疗杂志》2024年第10期1889-1892,1897,共5页Journal of Molecular Diagnostics and Therapy

基　　金：淄博市科学技术发展计划项目(2021kj062239)。

摘　　要：目的探讨2型糖尿病合并乙型肝炎肝硬化相关肝细胞癌患者血清肿瘤坏死因子-α(TNF-α)、热休克蛋白70(HSP70)、可溶性程序性细胞死亡蛋白1(sPD-1)的水平及意义。方法采用前瞻性研究方法,选取2022年1月至2023年6月98例2型糖尿病合并乙型肝炎肝硬化患者作为观察组,同时选取81例单纯性乙型肝炎肝硬化患者、单纯2型糖尿病患者60例、健康志愿者60名作为对照。根据是否合并肝细胞癌将观察组分为肝细胞癌组31例、非肝细胞癌67例组。检测所有研究对象血清TNF-α、HSP70和sPD-1水平。结果观察组血清TNF-α、HSP70明显高于乙肝肝硬化组、2型糖尿病组和健康志愿者,差异有统计学意义(P<0.05);观察组SPD-1明显低于乙肝肝硬化组、2型糖尿病组和健康志愿者,差异有统计学意义(P<0.05)。肝细胞癌组患者血清TNF-α、HSP70明显高于非肝细胞癌组,差异有统计学意义(P<0.05);肝细胞癌组患者血清SPD-1明显低于非肝细胞癌组,差异有统计学意义(P<0.05)。血清TNF-α、HSP70和sPD-1诊断2型糖尿病合并乙型肝炎肝硬化肝细胞癌的ROC曲线下面积(AUC)分别为0.812、0.749、0.848(P<0.01)。结论2型糖尿病合并乙型肝炎肝硬化相关肝细胞癌患者血清TNF-α、HSP70、sPD-1水平明显升高,在疾病诊治中可能有一定应用价值。Objective To investigate the serum levels of tumor necrosis factor-a(TNF-a),heat shock protein 70(HSP70)and soluble programmed cell death protein 1(sPD-1)in hepatocellular carcinoma(HCC)patients with hepatitis B cirrhosis and type 2 diabetes mellitus.Methods Prospective research methods were used,98 patients with type 2 diabetes mellitus and hepatitis B cirrhosis who were treated from January 2022 to June 2023 were selected as the observation group,and 81 patients with hepatitis B cirrhosis,60 patients with type 2 diabetes mellitus and 60 healthy volunteers were selected as controls.In addition,the observation group was divided into two subgroups:the hepatocellular carcinoma group with 31 cases and the non-hepatocellular carcinoma group,which had 67 cases.The serum levels of TNF-a,HSP70,and sPD-1 were then detected.Results The serum levels of TNF-a and HSP70 in the observation group were significantly higher than those in the hepatitis B cirrhosis group,the type 2 diabetes group and the healthy volunteers,the difference was statistically significant(P<0.05).The levels of sPD-1 in the observation group were significantly lower than those in the hepatitis B cirrhosis group,the type 2 diabetes group and the healthy volunteers,the difference was statistically significant(P<0.05).The serum levels of TNF-a and HSP70 in hepatocellular carcinoma group were significantly higher than those in the non-hepatocellular carcinoma group.The difference was statistically significant(P<0.05).The sPD-1 in the observation group was significantly lower than those in the non-hepatocellular carcinoma group.The difference was statistically significant(P<0.05).The area under the ROC curve(AUC)of serum TNF-a,HSP70,and sPD-1 in the diagnosis of type 2 diabetes mellitus complicated with hepatitis B cirrhosis and hepatocellular carcinoma were 0.812,0.749 and 0.848 respectively(P<0.01).Conclusion The serum levels of TNF-a,HSP70,and sPD-1 in hepatocellular carcinoma patients with hepatitis B cirrhosis and type 2 diabetes mellitus are significan

关 键 词：2型糖尿病 乙型肝炎肝硬化 肝细胞癌 肿瘤坏死因子-α 热休克蛋白70 可溶性程序性细胞死亡蛋白1 

分 类 号：R735.7[医药卫生—肿瘤] R587.2[医药卫生—临床医学]