一种婴幼儿汁类罐装辅助食品对幼龄小鼠急性炎症的影响及抗氧化活性评价  

Effects of a canned infant juice auxiliary food on acute inflammation in young mice and antioxidant activity

作　　者：戴志勇 邓斌 李佳妮 肖作为[1,3] 周一苗[1,3,4] DAI Zhiyong;DENG Bin;LI Jiani;XIAO Zuowei;ZHOU Yimiao(Homologous Innovation Laboratory of Medicine and Food,Hunan University of Traditional Chinese Medicine,Changsha,Hunan 410208,China;Engnice Holding Group Co.,Ltd.,Changsha,Hunan 410000,China;School of Pharmacy,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;Xiangyin Campus of Xiangxing College of Hunan University of Traditional Chinese Medicine,Yueyang,Hunan 414615,China)

机构地区：[1]湖南中医药大学药食同源创新实验室,湖南长沙410208 [2]英氏控股集团股份有限公司,湖南长沙410000 [3]湖南中医药大学药学院,湖南长沙410208 [4]湖南中医药大学湘杏学院,湖南岳阳414615

出　　处：《食品与机械》2024年第9期131-137,共7页Food and Machinery

基　　金：天然药物资源与功能开发基金项目(编号:2022ZYYGN06);湖南省中医药管理局重点项目(编号:C2023005)。

摘　　要：[目的]考察一种婴幼儿汁类罐装辅助食品对幼龄小鼠急性炎症的影响,并评价其抗氧化活性。[方法]将70只小鼠分为空白组、模型组、低剂量组(8.6979 g/kg)、中剂量组(17.3958 g/kg)、高剂量组(34.7916 g/kg)、超高剂量组(69.5832 g/kg)和阳性药物对照组,采用巴豆油致小鼠急性耳廓肿胀模型评价汁类罐装辅助食品对幼龄小鼠急性炎症的影响;计算除空白组外其余各组小鼠的左耳耳廓肿胀率;通过ELISA法检测其血清中TNF-α、IL-2、IL-6、IL-1β细胞因子含量;HE染色检测小鼠肝脏组织病理形态;通过电子舌进行滋味分析;采用紫外分光光度法测定汁类罐装辅助食品的抗氧化能力(DPPH自由基清除能力和羟自由基清除能力)。[结果]与空白组相比,模型组小鼠左耳肿胀明显,血清中TNF-α、IL-6、IL-1β含量明显上升(P<0.05),IL-2含量显著下降(P<0.05),肝组织中炎性细胞浸润明显。与模型组相比,低、中、高、超高剂量组小鼠左耳肿胀程度随剂量的升高而逐渐降低(P<0.05),血清中TNF-α、IL-6、IL-1β含量下降(P<0.05),且随着剂量的升高,下降程度增大。高剂量和超高剂量组小鼠的IL-2含量上升(P<0.05),炎症症状得到缓解;中、高、超高剂量组小鼠肝脏组织细胞核肿胀程度得到明显改善,弥漫性的炎性细胞浸染情况减轻;DPPH自由基清除率和羟自由基清除率的IC50分别为0.023,0.022 mg/mL(以样品中总酚计)。[结论]该种婴幼儿汁类罐装辅助食品对由涂抹巴豆油引起的幼龄小鼠急性炎症具有良好的抗炎效果。[Objective]To investigate the effects of an infant juice canned auxiliary food on acute inflammation and antioxidant activity in young mice.[Methods]70 mice were divided into blank group,model group,low dose group(8.6979 g/kg),middle dose group(17.3958 g/kg),high dose group(34.7916 g/kg),high dose group(69.5832 g/kg)and positive drug control group.Adopting croton oil-induced acute ear edema model in mice to evaluate the effects of juice canned supplementary food on acute inflammation in young mice;calculated the swelling rate of left ear in each group of mice except the blank group.The levels of TNF-α,IL-2,IL-6 and IL-1βin serum were detected by ELISA.HE staining was used to detect the pathological morphology of liver tissue in mice.Electronic tongue for taste analysis.The antioxidant capacity(DPPH free radical scavenging ability),·OH free radical scavenging ability of infant canned auxiliary food was determined by ultraviolet spectrophotometry.[Results]Compared with the blank group,the left ear swelling of the model group was obvious,the contents of TNF-α,IL-6 and IL-1βin serum were significantly increased(P<0.05),the content of IL-2 was significantly decreased(P<0.05),and the infiltration of inflammatory cells in liver tissue was obvious.Compared with the model group,the swelling degree of the left ear of the mice in the low,medium,high and ultra-high dose groups gradually decreased with the increase of the dose(P<0.05),and the contents of TNF-α,IL-6 and IL-1βin the serum decreased.The difference was statistically significant(P<0.05),and the degree of decrease increased with the increase of the dose.The content of IL-2 in the high-dose and ultra-high-dose groups of mice increased(P<0.05),and the inflammatory symptoms were alleviated.The results of HE staining showed that the degree of nuclear swelling in liver tissue of mice in the medium,high and ultra-high dose groups was significantly improved,and the diffuse inflammatory cell infiltration was reduced.The antioxidant results showed that the IC50 of DP

关 键 词：婴幼儿罐装辅助食品 急性炎症 抗氧化活性评价 

分 类 号：R153.1[医药卫生—营养与食品卫生学]