仿生支架与自体骨垂直骨增量的动物实验研究  

An experimental animal investigation of vertical bone augmentation utilizing biomimetic scaffolds and autogenous bone grafts

作　　者：王文洁 栗智 郝丽 刘超 周宏志 李玮 WANG Wen-jie;LI Zhi;HAO Li;LIU Chao;ZHOU Hong-zhi;LI Wei(Aerospace Center Hospital,Beijing 100049,China)

机构地区：[1]航天中心医院,北京100049

出　　处：《牙体牙髓牙周病学杂志》2024年第10期569-574,共6页Chinese Journal of Conservative Dentistry

基　　金：航天中心医院青年创新基金项目(2023QN05)。

摘　　要：目的:对比3D打印仿生多孔支架与自体骨块在兔颅骨垂直骨增量中的成骨效果。方法:仿真骨小梁设计多孔支架,并通过数字光3D打印技术获得生物活性玻璃仿生多孔支架。选择16只雄性壮年新西兰白兔,在兔颅骨顶植入4枚骨块移植材料,分别为来自双侧兔股骨髁的2枚自体骨与2枚3D打印多孔支架。移植骨块通过钛钉牢固固定,整体覆盖可吸收胶原膜,严密缝合。术后12周安乐死试验兔,获得兔颅骨样本,进行Micro-CT断层扫描分析并沿骨块直径长轴方向制作硬组织切片,进行影像学和组织学形态计量学分析。结果:2种材料植骨术区均实现了垂直骨增量。影像学分析显示多孔支架中新生骨高度(3.70±0.57)mm显著高于自体骨块(2.73±1.10)mm;P<0.05。组织形态学分析显示仿生多孔支架新生骨面积(15.28±3.36)mm^(2)显著高于自体骨块(6.46±1.98)mm^(2);P=0.002。结论:3D打印多孔生物活性玻璃仿生多孔支架具有更佳的空间维持力,在兔颅骨垂直骨增量中表现出更优的成骨效果。AIM:To compare the osteogenic effect between 3D printed simulated biomimetic scaffolds and autologous bone blocks in the vertical bone augmentation of rabbit calvarium.METHODS:Biomimetic scaffolds were designed with simulated trabecular bone,and bioactive glass porous scaffolds were obtained through digital light 3D printing technology.Sixteen male adult New Zealand white rabbits were selected,and 4 of bone graft materials were implanted on the top of the rabbit calvarium,namely 2 implants were autologous bones of bilateral rabbit femoral condyles and the other 2 implants derived from 3D printed porous scaffolds.The grafted bones were firmly fixed by titanium screws,covered with absorbable collagen membrane and sutured.The experimental rabbits were euthanized 12 weeks after the operation to obtain rabbit bone samples,and Micro-CT tomographic scanning analysis was performed and bone tissue sections were made along the long axis direction of the diameter of the bone blocks for imaging and histological morphometric analysis.RESULTS:Vertical bone augmentation was achieved in both bone grafting areas of the two materials.Micro-CT analysis showed that the height of new bone in the biomimetic scaffold(3.70±0.57)mm was significantly higher than that of the autologous bone block(2.73±1.10)mm,P<0.05.Histomorphological analysis showed that the area of new bone in the biomimetic scaffold(15.28±3.36)mm^(2) was significantly higher than that of the autologous bone block(6.46±1.98)mm^(2),(P=0.002).CONCLUSION:The 3D printed porous bioactive glass scaffold showed demonstrates superior space maintenance capability and enhanced osteogenic effects in vertical bone augmentation of rabbit skulls.

关 键 词：垂直骨增量 3D打印 生物活性玻璃 支架 牙种植 

分 类 号：R78[医药卫生—口腔医学]