基于Illumina Bovine SNP 50K芯片对比利时蓝牛的遗传规律解析  

Analysis of genetic law of Belgian blue cattle based on the illumina bovine SNP 50K chip

作　　者：王雪艳 李小鹏 韩亚辉 刘悦欣 陶雪雨 韩志鹏 杨睿智 张成龙 周稳 徐新如 刘书东[1,2] WANG Xueyan;LI Xiaopeng;HAN Yahui;LIU Yuexin;TAO Xueyu;HAN Zhipeng;YANG Ruizhi;ZHANG Chenglong;ZHOU Wen;XU Xinru;LIU Shudong(College of Animal Science and Technology,Tarim University,Alar 843300,China;Tarim Animal Science and Technology Key Laboratory,Xinjiang Production and Construction Corps,Alar 843300,China)

机构地区：[1]塔里木大学动物科学与技术学院,新疆阿拉尔843300 [2]新疆生产建设兵团塔里木畜牧科技重点实验室,新疆阿拉尔843300

出　　处：《黑龙江畜牧兽医》2024年第20期45-52,62,125,126,共11页Heilongjiang Animal Science And veterinary Medicine

基　　金：大学生创新创业训练计划项目(202210757004);新疆维吾尔自治区西门塔尔牛(安格斯)牛群体改良技术体系项目(2023XM-20)。

摘　　要：为了研究大型肉牛比利时蓝牛生长发育的遗传规律,筛选优异基因,试验基于Illumina Bovine SNP 50K芯片数据,采用PLINK软件对270头比利时蓝牛常染色体数据进行基因组长纯合片段(ROH)检测并基于选择信号分析,通过核苷酸多态性检测取前5%的单核苷酸多态性(single nucleotide polymorphism,SNP)位点,基于牛参考基因组(ARS-UCD1.2)对结果SNPs进行基因注释,对候选基因进行GO功能注释与KEGG信号通路富集分析,并计算染色体上ROH长度占基因组总长度的比例(FROH)。结果表明:在全部270个个体数据中共检测出1893个ROH片段,平均长度13.2311 Mb,平均FROH为0.0392;得到与生长发育相性状相关的基因有NEB、TET2、NEK11、NCKAP1、MYH15、EIF4A2、bta-miR-1248-1、DCAF8、PRORP、DOCK3、SYT15、MYEF2、ZDHHC13,与公牛生育能力相关的基因有CFAP61、DNAL1、BAG1。说明通过对比利时蓝牛生长发育性状相关分子标记的解析可以为比利时蓝牛遗传改良提供理论指导。In order to study the genetic rules of growth and development of large beef cattle,Belgian blue cattle,and screen excellent genes,based on the Ilumina Bovine SNP 50K chip data,PLINK software was used to detect the runs of homozygosity(ROH)in the genome of 270 Belgian blue cattle autosomal data and to conduct selective signal analysis.The top 5%SNP loci were selected by nucleotide polymorphism analysis,and the resulting SNPs were annotated using the bovine reference genome(ARS-UCD1.2).The candidate genes were enriched by GO functional annotation and KECG signaling pathway enrichment analysis,and the proportion of ROH length on the chromosome to the total genome length(FROH)was calculated.The results showed that a total of 1893 ROH segments were detected in all 270 individuals,with an average length of 13.2311 Mb and an average FROH of 0.0392.Genes associated with growth and development included NEB,TET2,NEK11,NCKAP1,MYH15,EIF4A2,bta-miR-1248-1,DCAF8,PRORP,DOCK3,SYT15,MYEF2 and ZDHHC13.Genes associated with bull fertility were CFAP61,DNAL1,and BAG1.The results indicated that the analysis of molecular markers related to the growth and developmental traits of Belgian blue cattle could provide theoretical guidance for the genetic improvement of Belgian blue cattle.

关 键 词：比利时蓝牛 Illumina Bovine SNP 50K芯片 长纯合片段 选择信号分析 基因注释 基因功能富集分析 

分 类 号：S823.2[农业科学—畜牧学]