基于SSR标记的菊苣种质资源DNA指纹图谱的构建  

Construction of DNA Fingerprints of Chicory(Cichorium intybus L.)Germplasm Resources Based on SSR Markers

作　　者：冯鹏龙 王丽慧[1] 杨世鹏[1] 孙雪梅[1] 侯志强[1] 李全辉[1] 钟启文[1] Feng Penglong;Wang Lihui;Yang Shipeng;Sun Xuemei;Hou Zhiqiang;Li Quanhui;Zhong Qiwen(Key Laboratory of Vegetable Genetics and Physiology,Academy of Agriculture and Forestry,Qinghai University,Xining,810016)

机构地区：[1]青海大学农林科学院,青海省蔬菜遗传与生理重点实验室,西宁810016

出　　处：《分子植物育种》2024年第21期7076-7084,共9页Molecular Plant Breeding

基　　金：青海省科学技术厅项目(2019-HZ-806;2021-ZJ-953Q);青海省蔬菜遗传与生理重点实验室项目(2020-ZJY02);青海省省级财政支农资金林草新技术推广项目(2020-SJTG-04)共同资助。

摘　　要：为挖掘国内外优质的菊苣种质资源,补充菊苣种质资源的DNA指纹图谱,为菊苣育种材料的选择以及新品种的鉴定提供技术支持。本研究以来源不同的23份菊苣资源为材料,采用SSR分子标记技术对这23份菊苣品种进行遗传多样性分析并构建分子指纹图谱。结果表明:通过对50对SSR引物进行PCR扩增,最终获得8对条带清晰稳定且多态性高的引物,占所用引物比例的16%。筛选出的8对SSR引物在23份菊苣种质资源中共扩增出82条条带,平均每对引物检测到10.25个等位基因,其中差异条带76条,多态性比率高达92.7%。扩增片段大小为50~500 bp之间,平均每个引物扩增出多态性条带9.5个,变化范围为6~11个,8对引物的多态性信息量PIC值范围为0.33~0.60,平均值为0.49;采用UPGMA法进行聚类,在GS 0.53处可将23份菊苣资源聚为5类;优选JJ13、JJ31、JJ125这3对引物组合便能准确快速区分23份菊苣种质资源。本研究的菊苣种质资源遗传多样性及构建的指纹图谱结果将为菊苣育种材料的选择、新品种的鉴定提供技术支持。In order to excavate high grade chicory in domestic and foreign germplasm resources,supplement the DNA fingerprint of chicory germplasm resources,provide technical support for the selection of chicory breeding materials and identification of new varieties.In this study,using 23 chicory resources from different sources as materials,SSR molecular marker technology was used to analyze the genetic diversity of 23 chicory and construct molecular fingerprints.The results showed that PCR amplification was performed in 50 pairs of SSR primers,and8 pairs of primers with clear and stable bands and high polymorphism were screened,accounting for 16%of the primers used.The selected 8 pairs of SSR primers amplified a total of 82 bands in 23 chicory germplasm resources,with an average of 10.25 loci detected per pair of primers,of which 76 were differential bands,and the polymorphism ratio was as high as 92.7%.The amplified fragment size ranged from 50 to 500 bp.On average,9.5 polymorphic bands amplified by each primer,and the variation range was 6~11.The PIC value of the polymorphism information of 8 pairs of primers ranges from 0.33 to 0.60,with an average of 0.49.Using UPGMA method,23chicory resources could be clustered into 5 categories at GS 0.53.Selecting three pairs of primer combinations of JJ13,JJ31 and JJ125 can accurately and quickly distinguish 23 chicory germplasm resources.The genetic diversity and fingerprint results of chicory germplasm resources in this study will provide technical support for the selection of chicory,breeding materials and the identification of new varieties.

关 键 词：菊苣 SSR标记 遗传多样性 DNA指纹图谱 

分 类 号：S636.9[农业科学—蔬菜学]