梨火疫病菌拮抗菌株组合的亲和力及其防病效果测定  

Determination of Affinity for Combination of Antagonistic Bacteria against Fire Blight Pathogen Erwinia amylovora and Their Biocontrol Effect

作　　者：宋淑敏 董代幸 盛强 韩剑[1,2] 罗明 SONG Shumin;DONG Daixing;SHENG Qiang;HAN Jian;LUO Ming(College of Agriculture,Xinjiang Agricultural University,Urumqi 830052,China;Key Laboratory for Prevention and Control of Invasive Organisms in Northwest Desert Oasis Agriculture and Forestry,Ministry of Agriculture and Rural Affairs,Urumqi 830052,China;Fuyang Center for Agricultural Technology Extension of Hangzhou City,Hangzhou 311400,China;Xinjiang Bazhou Academy of Agricultural Sciences,Korla Xinjiang 841003,China)

机构地区：[1]新疆农业大学农学院,农林有害生物检测与防控重点实验室,乌鲁木齐830052 [2]农业农村部西北荒漠绿洲农林外料入侵生物防控重点实验室,乌鲁木齐830052 [3]杭州市富阳区农业技术推广中心,杭州311400 [4]新疆巴音郭勒蒙古自治州农业科学研究院,新疆库尔勒841003

出　　处：《西北农业学报》2024年第11期2202-2211,共10页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家重点研发计划(2021YFD1400200);新疆维吾尔自治区自然科学基金重点项目(2021D01D12)。

摘　　要：为获得对梨火疫病菌(Erwinia amylovora)抑菌活性高、防病效果好的拮抗菌组合,以实验室前期从香梨酵素液中分离初筛出的E.amylovora拮抗细菌菌株为材料,采用对峙培养法检测菌株之间的亲和性,打孔-抑菌圈法测定复配组合菌株的抑菌活性。对筛选出相容性良好,对E.amylovora抑菌作用强的拮抗细菌组合通过喷施香梨离体花序和盆栽杜梨苗评价其对梨火疫病的防病效果。结果显示:筛选出相容性良好的3个拮抗细菌组合FX1-FJ20、FX74-FN12和FX70-FX43,其复配比例为1∶1的组合菌体发酵液和组合除菌发酵液的抑菌率均达到83%以上,明显高于单一菌株。3个拮抗细菌组合菌体发酵液和组合除菌发酵液对香梨花腐的3~7 d的平均预防型防效分别为56.26%~73.16%和72.02~82.66%;对杜梨苗的7~15 d的预防型防效分别为67.38%~77.73%和71.78%~80.11%,治疗型防效分别为64.53%~75.01%和69.09%~79.03%。组合菌的防效均较单个菌株提高,组合菌除菌发酵液的防效优于组合菌菌体发酵液,预防型防效优于治疗型防效。综合防效试验结果可见,拮抗菌组合FX74-FN12的防效最佳,其菌体发酵液和除菌发酵液对香梨花腐3~7 d的预防型防效分别为73.16%、82.66%,比单菌株的平均防效分别增加12.90%和22.40%;对杜梨苗的7~15 d的平均预防型和治疗型防效分别达到75.00%和79.00%以上,比单菌株的平均防效分别增加15.12%和11.02%,对梨火疫病具有较好的生防潜力。In the pursuit ofantagonistic bacterial combinations exhhibiting high inhibitory activity and efficacious disease prevention effect against Erwinia amylovora,strains isolated from the the fragrant pear enzyme solution at the early stage in the laboratory were used as materials.The affinity between these strains was assessed via plate confrontation culture,while their combined inhibitive activity were measured using punching-inhibition circle method.The antagonistic bacterial combination demonstrating substantial affinity and potent inhibition of E.amylovora were identified and further evaluated through in vitro spraying fragrant inflorescence and potted Birchleaf Pear seedlings.The results showed that combinations FX1-FJ20,FX74-FN12 and FX70-FX43,exhibited affinity and achieved inhibition rates more than 83%in both combined strains fermentation broth and combined strains filtered fermentation broth,significantly higher than that of the single strains.The average protective efficacy of the three antagonistic bacterial combinations on fragrant pear inflorescences ranged from 56.26%to 73.16%for combined strains fermentation broth and 72.02%to 82.66%for combined strains filtered fermentation broth over 3 to 7 days,similarly,on potted Birchleaf Pear seedlings,protective efficacy ranged from 67.38%to 77.73%for combined strains fermentation broth and 71.78%to 80.11%for combined strains filtered fermentation broth over 7 to 15 days,with therapeutic efficacy ranging from 64.53%to 75.01%and 69.09%to 79.03%,respectively.Overall,the combined strains consistently exhibited enhanced efficacy compared to the single strains,with filtered fermentation showing good results to fermentation alone in terms of protective and therapeutic effects.Particularly,combination FX74-FN12 demonstrated the highest efficacy,achieving protective efficacies of 73.16%and 82.66%in fermentation broth and filtered fermentation,respectively,during the initial 3-5 days against fragrant pear blossom rot-an increase of 12.90%and 22.40%,respectively,compare

关 键 词：梨火疫病菌 拮抗菌组合 相容性 抑菌活性 防效 

分 类 号：S476[农业科学—农业昆虫与害虫防治]