对藜高致病活性链格孢菌DT-DYLC的液体发酵培养基优化  

Liquid Medium Optimization of Alternaria alternata DT-DYLC with High Pathogenic Activity against Chenopodium album

作　　者：王宇哲 程海洋 程红玉 杨艳伶 伊国云 李娟 朱海霞[1,2] 魏有海 郭青云[1,2] 程亮 WANG Yuzhe;CHENG Haiyang;CHENG Hongyu;YANG Yanling;YI Guoyun;LI Juan;ZHU Haixia;WEI Youhai;GUO Qingyun;CHENG Liang(Qinghai Academy of Agricultural and Forestry Sciences,Qinghai University,Xining 810016,China;Qinghai Provincial Key Laboratory of Agricultural Integrated Pest Management,Xining 810016,China)

机构地区：[1]青海大学农林科学院,西宁810016 [2]青海省农业有害生物综合治理重点实验室,西宁810016

出　　处：《西北农业学报》2024年第11期2212-2221,共10页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：青海省自然科学基金(2022-ZJ-911)。

摘　　要：以链格孢菌(Alternaria alternata)DT-DYLC为研究对象,以生物量和孢子量(OD 600nm)为响应值,采用单因素试验和Plackett-Burman试验及响应面试验对其发酵培养基进行优化。结果表明,察氏固体培养基7 d时的菌落直径为73.1 mm,菌丝质量达0.049 g,均显著高于其他培养基。通过Plackett-Burman试验筛选出无机盐为影响孢子量的主要因素。运用响应面分析确定最优发酵培养基为:蔗糖68.853 g/L,NaNO_(3)11.41 g/L、K_(2) HPO_(4)5.523 g/L、KCl 1.405 g/L、MgSO_(4)·7H_(2) O 1.874 g/L、FeSO_(4)0.0375 g/L。在此条件下,孢子量达到3.4×10^(7) CFU/mL,是优化前的2.72倍。优化培养基可用于液体发酵工厂化生产。Using Alternaria alternata DT-DYLC as the research object,and with mycelium biomass and spore number(OD 600nm)as the response value,the fermentation medium was optimized by single factor test,Plackett-Burman test,and response surface test.The results showed that after 7 days of cultivation at 25°C on Chaw’s medium,the colony diameter and mycelium mass of DT-DYLC reached 73.1 mm,and the mycelial mass was 0.049 g,both significantly higher than other tested media.The main factors affecting the spore number of A.alternata DT-DYLC was inorganic salt.The optimal fermentation medium for spore production was determined by response surface methodology consisted of sucrose 68.853 g/L,NaNO_(3)11.41 g/L,K_(2) HPO_(4)5.523 g/L,KCl 1.405 g/L,MgSO_(4)·7H_(2) O 1.874 g/L,FeSO_(4)0.0375 g/L.Under these conditions,the spore number in the fermentation broth of A.alternata DT-DYLC reached 3.4×10^(7) CFU/mL,which was 2.72 times that of the unoptimized condition.The optimized medium can be used for the industrial-scale liquid fermentation of A.alternata DT-DYLC.

关 键 词：链格孢 孢子量 培养基优化 响应面法 

分 类 号：S451[农业科学—植物保护] TQ920.6[轻工技术与工程—发酵工程] TQ457