驼乳免疫球蛋白G对秀丽隐杆线虫的抗衰老作用  

Anti-aging Effect of Camel Milk Immunoglobulin G on Caenorhabditis elegans

作　　者：赵小萌 龚春淼 陈树兴[1,2] 王晓楠 李倩文 ZHAO Xiaomeng;GONG Chunmiao;CHEN Shuxing;WANG Xiaonan;LI Qianwen(College of Food and Bioengineering,Henan University of Science and Technology,Luoyang 471023,China;Food Laboratory of Zhongyuan,Luohe 462333,China)

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471023 [2]中原食品实验室,河南漯河462333

出　　处：《食品科学》2024年第22期112-117,共6页Food Science

基　　金：“十四五”国家重点研发计划重点专项(2023YFF1104505-5)。

摘　　要：目的:以秀丽隐杆线虫为模式生物,探究不同质量浓度(0.25、0.5、1、2、4 mg/mL)驼乳免疫球蛋白G(immunoglobulin G,IgG)对线虫的抗衰老作用。方法:通过对秀丽隐杆线虫寿命、繁殖能力、运动能力、抗应激能力及体内脂褐素水平等的分析测定,评价驼乳IgG的抗衰老活性。结果:与对照组相比,所喂食驼乳IgG质量浓度具备安全性,不会损伤线虫的生殖能力和运动能力;且在喂食质量浓度为1、2、4 mg/mL驼乳IgG时对线虫的平均寿命有显著延长作用;喂食0.5、4 mg/mL驼乳IgG时,线虫的吞咽频次显著提升(P<0.05);喂食0.25、0.5、1 mg/mL驼乳IgG时,线虫的抗热应激能力改善效果较为明显,其平均寿命得以显著延长(P<0.05);喂食1、4 mg/mL驼乳IgG时,线虫的抗氧化应激能力显著提升(P<0.05);喂食0.5、2 mg/mL驼乳IgG 14 d后,线虫体内脂褐素沉积显著降低(P<0.05)。结论:喂食一定质量浓度的驼乳IgG对线虫具有抗衰老的作用。Objective:To investigate the anti-aging effect of camel milk immunoglobulin G(IgG)at different concentrations(0.25,0.5,1,2,and 4 mg/mL)on the model organism Caenorhabditis elegans.Methods:The anti-aging activity of camel milk IgG was evaluated by analyzing the lifespan,reproductive capacity,locomotory capacity,stress resistance,and lipofuscin level of C.elegans.Results:Compared with the control group,the concentration of IgG in camel milk was safe,which damaged neither the reproductive nor locomotory capacity of C.elegans.Feeding of camel milk IgG at concentrations of 1,2,and 4 mg/mL significantly prolonged the average lifespan of C.elegans,and at 0.5 and 4 mg/mL significantly increased the swallowing frequency(P<0.05).Feeding of camel milk IgG at 0.25,0.5 and 1 mg/mL significantly improved the heat stress resistance,and significantly prolonged the average lifespan of C.elegans(P<0.05).Feeding of camel milk IgG at 1 and 4 mg/mL significantly increased the oxidative stress resistance of C.elegans(P<0.05),and lipofuscin deposition was significantly decreased after 14-day feeding of camel milk IgG at 0.5 and 2 mg/mL(P<0.05).Conclusion:Feeding a certain concentration of camel milk IgG has an anti-aging effect on C.elegans.

关 键 词：驼乳免疫球蛋白G 秀丽隐杆线虫 抗衰老 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程] TS201.4[轻工技术与工程—食品科学与工程]