miRNA-134与大脑中动脉重度狭窄性缺血性脑卒中患者MMP9的交互作用及对侧支循环形成的评估  

Interaction of miRNA-134 with MMP-9 in patients with severe middle cerebral artery stenosis ischemic stroke and assessment of collateral circulation formation

作　　者：陈秀晓 贾倩 董中君 Chen Xiuxiao;Jia Qian;Dong Zhongjun(Department of Neurology,Xingtai Center Hospital,Xingtai 054000,China)

机构地区：[1]邢台市中心医院神经内科,054000

出　　处：《中华保健医学杂志》2024年第5期585-588,共4页Chinese Journal of Health Care and Medicine

基　　金：邢台市重点研发计划项目(2020ZC280)。

摘　　要：目的探讨微小核糖核酸(miRNA)-134与大脑中动脉重度狭窄性缺血性脑卒中患者基质金属蛋白酶9(MMP9)的交互作用及对侧支循环形成的评估。方法前瞻性选取2021年8月~2022年8月于邢台市中心医院神经内科进行治疗的90例大脑中动脉重度狭窄性缺血性脑卒中患者作为观察组,其中,45例患者脑侧支循环不良,45例患者脑侧支循环良好;另选取同时期的90例健康人群作为对照组。收集患者的一般资料,测量患者的血清水平,使用受试者工作特征(ROC)曲线分析血清miRNA-134和MMP9水平对大脑中动脉重度狭窄性缺血性脑卒中患者的诊断价值,Pearson相关性分析血清中miRNA-134与MMP9的相关性,采用多因素logistic回归模型分析大脑中动脉重度狭窄性缺血性脑卒中患者脑侧支循环不良的影响因素。结果观察组的三酰甘油水平、收缩压均高于对照组,差异有统计学意义(t=12.743、21.940,P<0.05);观察组血清中的miRNA-134、同型半胱氨酸(Hcy)、超敏C-反应蛋白(hs-CRP)、MMP9水平均高于对照组,差异有统计学意义(t=14.862、23.229、21.649、20.868,P<0.05);ROC曲线分析结果显示,miRNA-134和MMP9均对重度缺血性脑卒中患者具有一定的诊断价值,AUC分别为0.875和0.812,而二者联合应用的诊断价值更高,AUC为0.942;Pearson相关性分析结果显示,大脑中动脉重度狭窄性缺血性脑卒中患者血清中miRNA-134的表达水平与MMP9呈正相关(r=0.783,P<0.05);多因素logistic回归分析结果显示,收缩压、三酰甘油、MMP9和miRNA-134均是大脑中动脉重度狭窄性缺血性脑卒中患者发生脑侧支循环不良的影响因素(P<0.05)。结论大脑中动脉重度狭窄性缺血性脑卒中患者血清中的miRNA-134、MMP9水平会出现明显的升高,而且两者存在相关性,能够为大脑中动脉重度狭窄性缺血性脑卒中患者发生脑侧支循环不良的诊断提供参考。Objective To explore the interaction between microRNA-134(miRNA-134)and Matrix Metalloproteinase-9(MMP-9)in patients with severe middle cerebral artery stenosis ischemic stroke and to assess the formation of collateral circulation.Methods A prospective study was conducted from August 2021 to August 2022,including 90 patients with severe middle cerebral artery stenosis ischemic stroke treated in the Department of Neurology at Xingtai Third Hospital,with 45 patients having poor collateral circulation and 45 having good collateral circulation;additionally,90 healthy individuals from the same period served as a control group.Patient demographic data were collected,and serum levels were measured.The diagnostic value of serum miRNA-134 and MMP-9 levels in patients with severe middle cerebral artery stenosis ischemic stroke was analyzed using the Receiver Operating Characteristic(ROC)curve.The correlation between miRNA-134 and MMP-9 in serum was analyzed using Pearson correlation,and a multivariate logistic regression model was used to analyze the factors affecting poor collateral circulation in these patients.Results The observation group had significantly higher levels of triglycerides and systolic blood pressure compared to the control group(t=12.743,21.940,P<0.05).Serum levels of miRNA-134,homocysteine(Hcy),high-sensitivity C-reactive protein(hs-CRP),and MMP-9 in the observation group were significantly higher than those in the control group(t=14.862,23.229,21.649,20.868,P<0.05).ROC curve analysis indicated that both miRNA-134 and MMP-9 have certain diagnostic value in patients with severe ischemic stroke,with AUCs of 0.875 and 0.812,respectively,and their combined application showed even higher diagnostic value,with an AUC of 0.942.Pearson correlation analysis revealed a positive correlation between the expression levels of miRNA-134 and MMP-9 in the serum of patients with severe middle cerebral artery stenosis ischemic stroke(r=0.783,P<0.05).Multivariate logistic regression analysis identified systolic blood press

关 键 词：缺血性脑卒中 基质金属蛋白酶9 侧支循环 

分 类 号：R743[医药卫生—神经病学与精神病学]