李氏禾内生细菌Bacillus cereus J01吸附Cr^(3+)的研究  

Study on adsorption of chromium(Cr^(3+))by endophytic bacteria Bacillus cereus J01 isolated from Leersia hexandra Swartz

作　　者：张泽宇 李子院[1] 王少杨 陈心怡 汪利琪 李海云[1] ZHANG Zeyu;LI Ziyuan;WANG Shaoyang;CHEN Xinyi;WANG Liqi;LI Haiyun(Guangxi Colleges and Universities Key Laboratory of Food Safety and Detection,College of Chemistry and Bioengineering,Guilin University of Technology,Guilin 541004,Guangxi,China)

机构地区：[1]桂林理工大学化学与生物工程学院,广西高校食品安全与检测重点实验室,广西桂林541004

出　　处：《广西植物》2024年第10期1839-1847,共9页Guihaia

基　　金：广西自然科学基金(2016GXNSFAA380014);国家自然科学基金(31460409)。

摘　　要：为考察李氏禾内生细菌蜡样芽孢杆菌Bacillus cereus J01菌株吸附Cr^(3+)的性能,以蜡样芽孢杆菌Bacillus cereus J01失活菌体作为生物吸附剂,采用单因素实验方法考察pH、温度、Cr^(3+)初始浓度、吸附剂用量、吸附时间等条件对Cr^(3+)吸附性能的影响,分析其等温吸附过程、动力学过程及热力学过程,并采用红外光谱方法对吸附机理进行初步分析。结果表明:(1)在50 mL反应体系中,当pH值为6、温度为40℃、Cr^(3+)初始浓度为150 mg·L^(-1)、吸附剂投加量为0.2 g、吸附时间为12 h时,蜡样芽孢杆菌Bacillus cereus J01失活菌体对Cr^(3+)的吸附性能达到最佳,其平衡吸附量和Cr^(3+)去除率分别为34.30 mg·g^(-1)、91.60%。(2)等温吸附过程分析结果显示,朗格缪尔吸附等温模型能更好地模拟蜡样芽孢杆菌Bacillus cereus J01失活菌体对Cr^(3+)的吸附,其吸附过程更倾向于单分子层吸附。(3)吸附过程动力学分析结果显示,蜡样芽孢杆菌Bacillus cereus J01失活菌体对Cr^(3+)的吸附更符合准二级动力学速率方程。(4)吸附过程热力学分析结果显示,在40℃下,吸附过程的△G、△H和△S分别为-2.609 kJ·mol^(-1)、61.792 kJ·mol^(-1)和206.11 J·mol^(-1),该温度下吸附过程是自发过程。(5)红外光谱分析结果显示,蜡样芽孢杆菌Bacillus cereus J01失活菌体对Cr^(3+)的吸附可能通过细胞成分中的氨基、羟基和羰基起作用。该研究结果表明该菌的失活菌体对Cr^(3+)具有较强的吸附性能,并在铬污染的环境治理中具有较好的应用潜力。The effects of pH,temperature,initial Cr^(3+)concentration,adsorbent dosage,time,and other factors on the adsorption capacity of Cr^(3+)were examined by the single factor test method using inactivated Bacillus cereus J01 strain as biological adsorbent in order to investigate the adsorption performance of Cr^(3+)by endophytic bacterium Bacillus cereus J01 from Leersia hexandra Swartz.The kinetic,thermodynamic,and isothermal adsorption processes were analyzed.Infrared spectroscopy was used to provide a preliminary analysis of the Cr^(3+)adsorption mechanism.The results were as follows:(1)In the reaction system of 50 mL,inactivated Bacillus cereus J01 reached the best adsorption performance on Cr^(3+)when pH value was 6,temperature was 40℃,initial concentration of Cr^(3+)was 150 mg·L^(-1),adsorbent dosage was 0.2 g and adsorption time was 12 h.The equilibrium adsorption capacity was 34.30 mg·g^(-1) and the removal rate of Cr^(3+)was 91.60%.(2)The Langmuir isothermal adsorption model could better simulate the adsorption of Cr^(3+)by inactivated Bacillus cereus J01.The adsorption process was more similar to monolayer adsorption.(3)The adsorption kinetics analysis showed that the adsorption of inactivated Bacillus cereus J01 on Cr^(3+)was more consistent with quasi-second-order kinetic rate equation.(4)Thermodynamic analysis of adsorption showed that at 40℃,△G,△H and△S were-2.609 kJ·mol^(-1),61.792 kJ·mol^(-1) and 206.11 J·mol^(-1),respectively.The adsorption process was spontaneous at 40℃.(5)The results of infrared spectroscopy showed that the adsorption of inactivated Bacillus cereus J01 on Cr^(3+)could be attributed to the effects of amino,hydroxyl and carbonyl groups in the cell components.The results show that the inactivated bacteria of this bacterium has strong adsorption capacity for Cr^(3+),and has good application potential in the treatment of environmental chromium pollution.

关 键 词：蜡样芽孢杆菌 吸附 Cr^(3+) 李氏禾 内生细菌 

分 类 号：Q946[生物学—植物学]