酵母β-葡聚糖对玉米赤霉烯酮诱导小鼠睾丸细胞铁死亡的缓解作用  

Alleviation of zearalenone-induced ferroptosis in mouse testicular cells by yeast beta-glucan

作　　者：赵子惠 李佳容 常倩雯 朱琪 张亚宁 胡婉星 贺俊平[1] ZHAO Zihui;LI Jiarong;CHANG Qianwen;ZHU Qi;ZHANG Yaning;HU Wanxing;HE Junping(College of Veterinary Medicine,Shanxi A gricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学动物医学学院,山西太谷030801

出　　处：《中国兽医科学》2024年第10期1408-1416,共9页Chinese Veterinary Science

基　　金：山西省现代农业牛产业技术体系项目(2023CYJSTX13-03);山西农业大学农业发展科技引领项目(CYYL-08)。

摘　　要：为探究玉米赤霉烯酮(ZEA)对雄性小鼠铁死亡相关基因在睾丸组织中表达和定位的影响,以及β-葡聚糖对ZEA诱导小鼠睾丸细胞铁死亡的缓解作用。随机将50只雄性小鼠分为对照组(灌胃玉米油)、ZEA组(LG:0.5 mg/kg、MG:5 mg/kg、HG:50 mg/kg)和β-葡聚糖组(TG:50 mg/kg ZEA+50 mg/kgβ-葡聚糖),连续灌胃10 d。用台盼蓝染色法、HE染色、免疫组化、Western-blot和qRT-PCR等方法检测小鼠精子和睾丸结构的变化及铁死亡基因GPX4、SLC7A11和FTH1的表达与定位。结果显示,与对照组相比,ZEA组畸形精子增多、曲精小管内生精细胞排列紊乱,睾丸组织Fe含量升高(P<0.01)、MDA含量升高(P<0.001)、SOD含量下降(P<0.001),GPX4、SLC7A11和FTH1的表达降低(P<0.05),GPX4在精原细胞的细胞核表达,SLC7A11在精原细胞和精母细胞的细胞核表达,FTH1在精原细胞的细胞核和细胞膜表达。β-葡聚糖组与ZEA组相比畸形精子减少、生精细胞排列有序,Fe含量降低(P<0.05)、MDA含量降低(P<0.01)、SOD含量上升(P<0.001),GPX4、SLC7A11和FTH1的表达升高(P<0.05)。结果表明,ZEA可以通过降低GPX4、SLC7A11和FTH1在生精细胞的表达引起睾丸细胞铁死亡,酵母β-葡聚糖对ZEA诱导的小鼠睾丸细胞铁死亡有缓解作用。To investigate the effects of zearalenone(zEA)on the expression and localization of ferroptosis-related genes in testicular tissues of male mice and the mitigating effects ofβ-glucan on zEA-induced ferroptosis in mouse testicular cells.Fifty male mice were randomly divided into control group(gavaged with corn oil),ZEA group(LG:0.5 mg/kg,MG:5 mg/kg,HG:50 mg/kg)andβ-glucan group(TG:50 mg/kg ZEA+50 mg/kgβ-glucan)for 10 days.Taipan blue staining,HE staining,immunohistochemistry,Western-blot and qRT-PCR were used to detect changes in the structure of mouse spermatozoa and testes and the expression and localization of the ferroptosis genes GPX4,SLC7A1l and FTH1.Compared with the control group,the zEA group showed increased malformed spermatozoa,disorganized spermatogenic cells in the seminiferous tubules,elevated Fe content(P<0.01)and MDA content(P<0.001),decreased SOD content(P<0.001),and decreased expression of GPX4,SLC7A11,and FTH1 in the testicular tissues(P<0.05).GPX4 was expressed in the nucleus of spermatogonia,SLC7A1l was expressed in the nu-cleus of spermatogonia and spermatocytes,and FTH1 was expressed in the nucleus and cell membrane of spermatogonia.Compared with ZEA group,β-glucan group had fewer abnormal sperm and more orderly spermatogenic cells,Fe content(P<0.05),MDA content(P<0.01)decreased,S0D content increased(P<0.001),and the expression of GPX4,SLC7A11,and FTH1 was elevated(P<0.05).The results showed that ZEA could cause testicular cell iron death by decreasing the expression of GPX4,SLC7A1l and FTH1 in spermatogenic cells,and that yeastβ-glucan had an attenuating effect on ZEA-induced testicular cell ferroptosis inmice.

关 键 词：玉米赤霉烯酮 铁死亡 Β-葡聚糖 睾丸细胞 小鼠 

分 类 号：S856.9[农业科学—临床兽医学]