4种混合光合厌氧产氢菌群的筛选及产氢性能研究  

SCREENING AND HYDROGEN PRODUCTION PERFORMANCE OF FOUR MIXED PHOTOSYNTHETIC ANAEROBIC HYDROGEN-PRODUCING BACTERIAL FLORA

作　　者：黄加亮 穆兰 武婉婷 彭皓 陶俊宇 宋英今[4] 石岩[3] 陈冠益 HUANG Jialiang;MU Lan;WU Wanting;PENG Hao;TAO Junyu;SONG Yingjin;SHI Yan;CHEN Guanyi(The College of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134,China;The College of Mechanical Engineering,Tianjin University of Commerce,Tianjin 300134,China;Interdisciplinary Innovation Lab for Environment and Energy,Tianjin University of Commerce,Tianjin 300134,China;The College of Environmental Science and Engineering,Tianjin University,Tianjin 300350,China)

机构地区：[1]天津商业大学生物技术与食品科学学院,天津300134 [2]天津商业大学机械工程学院,天津300134 [3]天津商业大学环境能源+X创新实验室,天津300134 [4]天津大学环境科学与工程学院,天津300350

出　　处：《环境工程》2024年第9期301-310,共10页Environmental Engineering

基　　金：国家重点研发项目“生物质干式厌氧消化制气与沼渣液综合处置利用关键技术”(2022YFE0125100);国家自然科学基金项目“金属掺杂碳量子点调控厨余垃圾暗发酵液光合产氢机制研究”(52200169);天津市科技计划项目“PLA废塑料与秸秆联合低温水热强化厌氧产甲烷的协同机制研究”(22JCQNJC01410)。

摘　　要：为实现可持续绿色能源目标,将来源广泛的生物质能源高效资源化,采用暗光混合发酵技术,从好氧污泥、厌氧污泥、湖泥、牛粪中富集了4种暗光混合菌群利用葡萄糖进行厌氧发酵制氢实验,并对发酵过程中微生物、产氢、生长、物质变化特性进行研究。16S rRNA高通量结果显示,4种菌群均是以红假单胞菌属(Rhodopseudomonas)为主的暗光混合菌群,菌群结构具有多样性,菌群之间差异明显。其中,牛粪混合菌群产氢性能最好,在30 mmol/L葡萄糖、35℃、5000 lux条件下,累积氢气产率为(633.93±4.87)mL/L,最大产氢速率为(8.55±0.31)mL/(L·h),能量转化率可达到9.22%,底物中7.32%的电子流向H_(2)。结果表明:暗光混合菌群能高效地利用底物进行厌氧发酵制氢。The efficient utilization of biomass energy from a wide range of sources is beneficial to achieving the goal of sustainable green energy.In this study,the dark light mixed fermentation technology was used to enrich four kinds of dark light mixed bacterial flora from aerobic sludge,anaerobic sludge,lake mud and cow dung,and the anaerobic fermentation of glucose was used to produce hydrogen.In this study,microorganisms,hydrogen production,growth,and material change characteristics during fermentation were investigated.The 16S rRNA high-throughput results showed that all four flora were dark-light mixed colonies dominated by the genus Rhodopseudomonas sp.,with a diversity of flora structures,and obvious differences among the four flora.Among the four dark-light hybrid flora,the hydrogen production performance of the cow dung hybrid flora was the best,with a cumulative hydrogen yield of(633.93±4.87)mL/L,a maximum hydrogen production rate of(8.55±0.31)mL/(L·h),and an energy conversion rate of 9.22%,with 7.32%of the electrons in the substrate flowed to the hydrogen,under the condition of 30 mmol/L glucose,35℃,and 5000 lux.The results showed that the dark-light hybrid flora could efficiently utilize the substrate for hydrogen production by anaerobic fermentation.

关 键 词：生物制氢 暗光耦合发酵 暗光混合菌群 光合细菌 能量转化效率 电子转移 

分 类 号：TQ116.29[化学工程—无机化工] TQ920.6[轻工技术与工程—发酵工程]