恰玛古多糖含量测定方法的优化  

Optimization of the method for the determination of polysaccharide content in Brassica rapa L.

作　　者：许璐 伊木然江·苏布哈提 谢军 王苗苗 罗洁 李慕春 XU Lu;YIMURANJIANG Su-Bu-Ha-Ti;XIE Jun;WANG Miao-Miao;LUO Jie;LI Mu-Chun(Xinjiang Academic Institute of Analysis and Testing,Urumqi 830011,China;Autonomous Region Market Supervision and Administration Audit and Evaluation Center,Urumqi 830063,China)

机构地区：[1]新疆维吾尔自治区分析测试研究院,乌鲁木齐830011 [2]新疆维吾尔自治区市场监督审核评价中心,乌鲁木齐830063

出　　处：《食品安全质量检测学报》2024年第18期252-262,共11页Journal of Food Safety and Quality

基　　金：自治区重点研发专项计划项目(2022B02037-3);青年拔尖人才项目—青年科技创新人才项目(2023TSYCCX0074)。

摘　　要：目的将传统的苯酚-硫酸法和3,5-二硝基水杨酸(3,5-dinitrosalicylic acid,DNS)法相结合,建立恰玛古多糖含量测定的方法。方法采用单因素实验和Box-Behnken实验设计的响应面工艺,筛选出测定过程中苯酚-硫酸法和DNS法的最佳条件组合。结果总糖测定时,反应时间设为27.22 min,苯酚试剂与硫酸的用量分别为0.32 mL和1.08 mL;而在还原糖测定中,显色反应时间优化为20.53 min,DNS显色剂用量为2.08 mL,并需静置14.90 min以确保充分反应。利用上述确定的最佳条件测定的恰玛古多糖含量为36.4732 g/100 g。结论本研究通过精密度、重复性、稳定性实验对测量结果进行考察表明,苯酚-硫酸法和DNS组合方法不仅有效解决了传统方法中的干扰问题,还确保了测量结果的可靠性与有效性。本研究的成功实施,不仅弥补了恰玛古多糖含量测定领域的空白,也为相关科研工作者提供了科学、准确的测定方法,有望推动恰玛古多糖研究领域的深入发展。Objective To combine the traditional sulfuric acid-phenol method and 3,5-dinitrosalicylic acid(DNS)method to establish an approach for the determination of the polysaccharide content.Methods Used the response surface process designed by Box-Behnken experiments to screen the optimal combination of conditions for the phenol-sulfuric acid and DNS methods in the measurement process.Results The optimal conditions in the total sugar content determination method were a reaction time of 27.22 min,a phenol reagent dosage of 0.32 mL,and a sulfuric acid dosage of 1.08 mL,The optimal conditions for the determination of reducing sugars were 20.53 min for the color development reaction time,2.08 mL for the color developer DNS,and 14.90 min for the resting time.The content of chamagogic polysaccharides determined using the optimum conditions determined above was 36.4732 g/100 g.Conclusion Through rigorous precision,repeatability,and stability experiments,the assessment of measurement results demonstrates that the combined method of sulfuric acid-phenol and DNS not only effectively addresses the interference issues inherent in traditional methods but also ensures the reliability and validity of measurement outcomes.This provides a solid technical foundation for the quantitative study of Brassica rapa L.polysaccharide content.The successful implementation of this study not only fills the gap in the field of Brassica rapa L.polysaccharide content determination but also offers scientific and accurate measurement methods to relevant researchers,potentially fostering further advancements in the research of Brassica rapa L.polysaccharides.

关 键 词：恰玛古 多糖 苯酚-硫酸法 3 5-二硝基水杨酸法 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]