液相色谱法测定婴幼儿辅食营养包中维生素B2  

Determination of vitamin B2 in infant complementary food package using HPLC

作　　者：朱春燕 王津 刘哲宇 陈文彬 刘星艳 刘琳 钟利敏 ZHU Chunyan;WANG Jin;LIU Zheyu;CHEN Wenbin;LIU Xingyan;LIU Lin;ZHONG Limin(Ganzhou Quanbiao Biological Technology Co.,Ltd.,Ganzhou 341100)

机构地区：[1]赣州市全标生物科技有限公司,赣州341100

出　　处：《中国食品添加剂》2024年第11期208-212,共5页China Food Additives

摘　　要：在酸性条件下采用乙腈-1%乙酸溶液(5+95)超声提取婴幼儿辅食营养包中的维生素B2,用液相色谱仪定性,外标法定量,建立一种快速测定婴幼儿辅食营养包中维生素B2的高效液相色谱检测方法。结果表明,维生素B2在0.20~4.0μg/mL范围内呈良好的线性关系,线性相关系数为0.9998;在5.0、10.0和50.0 mg/kg浓度水平下,添加回收率范围为92.32%~103.25%,相对标准偏差RSD(n=6)为0.46%~4.98%,方法定量限为5.0 mg/kg。A rapid and efficient High performance liquid chromatography method for determining vitamin B2 in infant complementary food backage was established.Vitamin B2 in infant complementary food backage samples were extracted with acetonitrile 1%,acetic acid solution(5+95)by ultrasound,qualitatively analyzed by liquid chromatography,and quantified by external standard method.The results showed that vitamin B2 exhibited a good linear relationship in the range of 0.20 to 4.0μg/mL,with a correlation coefficient of 0.9998,and recovery rate ranged from 92.32%to 103.25%at the levels of 5.0,10.0 and 50.0 mg/kg.The relative standard deviation RSD was 0.46%〜4.98%(n=6)and the quantitative limit of the method was 5.0 mg/kg.

关 键 词：维生素B2 婴幼儿辅食营养包 高效液相色谱法 

分 类 号：TS202.3[轻工技术与工程—食品科学] O657.72[轻工技术与工程—食品科学与工程]