PFKP在肺腺癌组织中的表达及其与预后的关系  

The expression of PFKP in lung adenocarcinoma tissue and its relationship with prognosis

作　　者：田喆 岑丽兰 韦枫[1,2] 邓俊华[1,2] 覃春艳[1,2] 王志波 许世阳 董珏 黄玉兰[1,2] 蒋玉洁[1,2] TIAN Zhe;CEN Lilan;WEI Feng;DENG Junhua;QIN Chunyan;WANG Zhibo;XU Shiyang;DONG Jue;HUANG Yulan;JIANG Yujie(Department of Pulmonary and Critical Care Medicine,the Affiliated Hospital of Youjiang Medical University for Nationalities,Guangxi Baise 533000,China;Life Science and Clinical Medicine Research Center,the Affiliated Hospital of Youjiang Medical University for Nationalities,Guangxi Baise 533000,China;Guangxi Academy of Medical Sciences,Department of Infectious Disease,the People's Hospital of Guangxi Zhuang Autonomous Region,Guangxi Nanning 530021,China)

机构地区：[1]右江民族医学院附属医院呼吸与危重症学科,广西百色533000 [2]右江民族医学院附属医院生命科学与临床医学研究中心,广西百色533000 [3]广西医学科学院广西壮族自治区人民医院感染性疾病科,广西南宁530021

出　　处：《现代肿瘤医学》2024年第22期4296-4304,共9页Journal of Modern Oncology

基　　金：国家自然科学基金(编号:81860021);广西自然科学基金(编号:2021GXNSFAA325003);广西壮族自治区卫生和计划委员会自筹经费科研课题(编号:Z20180216);广西高校中青年教师科研基础能力提升项目(编号:2023KY0573);广西省百色市区域多发病联合专项计划(编号:百科字[2022]41号);广西省百色市科学研究与技术开发项目(编号:百科20232086)。

摘　　要：目的:探索磷酸果糖激酶血小板型(platelet-type phosphofructokinase,PFKP)在肺腺癌(adenocarcinoma of lung,LUAD)中的关键作用。方法:利用癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库观察PFKP在肺腺癌组织和癌旁组织中的表达情况。采用免疫组化检测临床样本肺腺癌组织和癌旁组织PFKP表达情况。利用TCGA数据库进一步探讨PFKP的表达及其在LUAD预后和免疫浸润中的作用。结果:PFKP在LUAD中高表达,且PFKP高表达与临床病理特征(AJCC分期和TNM分期)及不良预后相关。Kaplan-Meier生存分析和ROC曲线分析进一步证实,PFKP高表达组患者的中位总生存时间显著低于低表达组,且在1年、3年和5年生存预测中呈现出高度预测性。富集分析表明,PFKP的生物学功能参与到抗肿瘤药物代谢的过程中。此外PFKP与肿瘤微环境和免疫治疗密切相关。本研究筛选出一批对PFKP高表达的LUAD患者敏感性较高的临床药物和正在被研究的抑制剂。结论:PFKP在LUAD发生发展中的关键作用和其作为潜在药物治疗靶标的可能性,使其成为肺癌研究和治疗的重要靶标。Objective:To investigate the pivotal role of platelet-type phosphofructokinase(PFKP)in adenocarcinoma of lung(LUAD).Methods:PFKP expression in LUAD tissues and adjacent normal tissues was assessed utilizing The Cancer Genome Atlas(TCGA)database.In addition,immunohistochemistry was conducted on clinical samples of LUAD tissues and adjacent normal tissues to evaluate PFKP expression.The TCGA database was further exploited to investigate PFKP expression and its correlation with LUAD prognosis and immune infiltration.Results:Our findings unveiled upregulated PFKP expression in LUAD tissues,established an association with clinical pathological features(AJCC stage and TNM stage)and poor prognosis.Kaplan-Meier survival analysis and ROC curve analysis substantiated these observations by demonstrating that patients with high PFKP expression exhibited shorter median overall survival than those with low expression.Notably,PFKP expression displayed heightened predictive value for 1-year,3-year,and 5-year survival predictions.Enrichment analysis disclosed the involvement of PFKP's biological functions in anti-tumor drug metabolism processes.Moreover,PFKP exhibited close associations with the tumor microenvironment and immune therapy.Consequently,our study identified several clinical drugs and inhibitors that exhibited increased sensitivity in LUAD patients with high PFKP expression.Conclusion:PFKP's critical role in the onset and progression of LUAD and its potential as a drug therapy target make its significance in both research and treatment.

关 键 词：肺腺癌 PFKP 肿瘤分期 免疫治疗 肿瘤微环境 

分 类 号：R734.2[医药卫生—肿瘤]