血小板、凝血因子、内皮素与脑梗死患者脑血栓形成相关性研究  

Study on the correlation between platelet,coagulation factor and endothelin levels with cerebral thrombosis in patients with cerebral infarction

作　　者：汤俊峰 邵枝定 唐晓磊 TANG Junfeng;SHAO Zhiding;TANG Xiaolei(Department of Neurology,the Second Affiliated Hospital of Wannan Medical College,Wuhu 241000,China;Basic Laboratory,the Second Affiliated Hospital of Wannan Medical College,Wuhu 241000,China)

机构地区：[1]皖南医学院第二附属医院神经内科,安徽芜湖241000 [2]皖南医学院第二附属医院基础实验室,安徽芜湖241000

出　　处：《长春中医药大学学报》2024年第11期1269-1273,共5页Journal of Changchun University of Chinese Medicine

基　　金：安徽省重点研究与开发计划项目(S202104j07020044)。

摘　　要：目的研究血小板、凝血因子、内皮素水平与脑梗死患者脑血栓形成的相关性及鉴别价值。方法前瞻性选取2023年1月-2023年12月来皖南医学院第二附属医院诊治的120例有新发脑血栓形成的脑梗死患者设为观察组,另选同时间段收入治疗的无新发脑血栓形成的脑梗死患者112例为对照组。采集受试者肘检测静脉血5 mL,离心取上清液,检测血小板膜糖蛋白P-选择素(CD62P),血小板(PLT)、凝血酶原时间(PT)、活化部分凝血活酶时间(APTT);采用酶联免疫吸附试验(ELISA)试剂盒检测内皮素(ET)等指标。分析以上指标对脑梗死的诊断效能,分析其对美国国立卫生研究院卒中量表(NIHSS)的影响。结果观察组临床指标改善均优于对照组,差异具有统计学意义(P<0.05);ET、Ⅷ因子水平、PLT、CD62P、NIHSS、C-反应蛋白(CRP)两两之间呈正相关关系(P<0.05),但均与PT、APTT呈负相关关系(P<0.05);影响NIHSS的因素有分组(对象属性)、高血压、Ⅷ因子水平、PLT、CD62P、CRP。鉴别价值:AUC:ET>Ⅷ因子水平>CRP>NIHSS>PLT(Z=4.000,P=0.406);敏感度:PLT>ET>22 CRP>Ⅷ因子水平>NIHSS(χ^(2)=0.367,P=0.545);特异性:Ⅷ因子水平>NIHSS>CRP>ET>PLT(χ^(2)=0.045,P=0.832)。结论血小板、凝血因子、内皮素水平均可用于脑梗死患者脑血栓形成的诊断鉴别,且两两之间具有正相关关系。Objective To study the correlation and differential value of platelet,coagulation factor and endothelin levels with cerebral thrombosis in patients with cerebral infarction.Methods A total of 120 cerebral infarction patients with newly developed cerebral thrombosis who were diagnosed and treated in the Second Affiliated Hospital of Wannan Medical College from January 2023 to December 2023 were prospectively selected and set as the observation group,and in addition,112 cerebral infarction patients without newly developed cerebral thrombosis who were admitted to the same hospital during the same period were selected as the control group.5 mL of venous blood was collected from the elbow of the subjects,and the supernatant was collected by centrifugation to detect platelet membrane glycoprotein P-selectin(CD62P)and platelets(PLT)levels,prothrombin time(PT),and activated partial thromboplastin time(APTT).Enzyme-linked immunosorbent assay(ELISA)kit was used to detect the levels of endothelin(ET)and other indicators.The diagnostic efficacy of the above indicators for cerebral infarction was analyzed,and their impact on National Institute of Health Stroke Scale(NIHSS)was analyzed.Results The improvement of clinical indicators in the observation group was better than that in the control group,and there was statistically significant difference(P<0.05);The levels of ET,factor VIII,PLT,CD62P and C-reactive protein(CRP),and NIHSS were positively correlated with each other(P<0.05),but negatively correlated with PT and APTT(P<0.05).The factors that affected NIHSS included grouping(object attributes),hypertension,and the levels of factor VIII,PLT,CD62P and CRP.Differential value:AUC:ET>factor VIII level>CRP>NIHSS>PLT(Z=4.000,P=0.406);Sensitivity:PLT>ET>CRP>factor VIII level>NIHSS(χ^(2)=0.367,P=0.545);Specificity:Factor VIII level>NIHSS>CRP>ET>PLT(χ^(2)=0.045,P=0.832).Conclusion The levels of platelets,coagulation factors and endothelin can all be used for the diagnosis and differentiation of cerebral thrombosis in patients wit

关 键 词：血小板 凝血因子 内皮素 脑梗死 相关性 鉴别价值 

分 类 号：R743.33[医药卫生—神经病学与精神病学]