鹳榆止泻散防治猪溃疡性结肠炎的潜在作用机制  

Potential Mechanism of Guanyu Zhixie Powder in Control of Porcine Ulcerative Colitis

作　　者：马挺 纪鹏[1] 魏彦明[1] 李琛琛 武凡琳 华永丽[1] 张文飞 王祈帆 杨昊赤 MA Ting;JI Peng;WEI Yanming;LI Chenchen;WU Fanlin;HUA Yongli;ZHANG Wenfei;WANG Qifan;YANG Haochi(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学动物医学院,兰州730070

出　　处：《西南大学学报（自然科学版）》2024年第11期67-80,共14页Journal of Southwest University(Natural Science Edition)

基　　金：甘肃省高校产业支撑项目(2020C-14);甘肃农业大学青年导师基金项目(GAU-QDFC-2021-04);甘肃农业大学伏羲英才项目(Gaufx-02Y05);财政部和农业农村部国家现代农业产业技术体系项目(CARS-37);中国科学院兰州化学物理研究所技术委托项目(GSAU-JSYF-2021-021)。

摘　　要：采用网络药理学和分子对接技术探究自拟中药组方鹳榆止泻散防治猪溃疡性结肠炎(PUC)的作用靶点及机制.通过中药系统药理学数据库与分析平台(TCMSP)筛选鹳榆止泻散中5种药物活性成分及靶点,基于PUC相关疾病数据库(GeneCards,OMIM,PharmGkb,TTD,DrugBank)综合检索疾病相关靶点,Venn可视化两者共同关键靶点.采用STRING平台构建潜在靶点蛋白互作(PPI)网络,Cytoscape 3.8.0软件对关键靶点进行网络拓扑学分析及核心基因提取,同时建立鹳榆止泻散“活性成分-潜在作用靶点”网络,并对所得的核心基因进行基因本体(GO)及京都基因与基因组百科全书(KEGG)富集分析,预测鹳榆止泻散治疗PUC的分子机制,最后通过Autodock软件进行结合位点和分子对接验证.通过数据分析得出鹳榆止泻散治疗PUC可能与槲皮素、苦参碱、山奈酚、β-谷甾醇、柚皮素、木犀草素、甘草异黄酮等化学成分有关,筛选出fos,jun,rela,esr1,mapk1等为核心靶点,富集分析得到GO条目2 461条,KEGG通路176条,主要涉及炎症信号通路和PI3K/Akt信号通路等,分子对接结果显示关键活性成分与对应靶点具有较好的结合活性.结果表明:鹳榆止泻散中活性成分通过干预fos,jun,rela,esr1,mapk1等关键靶点,调节多条信号通路,抑制炎症反应,发挥防治猪溃疡性结肠炎的效果.To explore the target and mechanism of Guanyu Zhixie Powder in the prevention and treatment of porcine ulcerative colitis(PUC) by network pharmacology and molecular docking technology,the active components and targets of five active ingredients in Guanyu Zhixie Powder were screened by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).Disease-related targets were comprehensively searched based on PUC-related disease databases(GeneCards,OMIM,PharmGkb,TTD,DrugBank),and the common key targets of the two were visualized by Venn.The STRING platform was used to construct a potential target protein interaction(PPI) network.Cytoscape 3.8.0 software was used to perform network topology analysis and core gene extraction on key targets.At the same time,the ‘active ingredient-potential target gene' network of Guanyu Zhixie Powder was established.The obtained core genes were subjected to gene ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analysis to predict the molecular mechanism of Guanyu Zhixie Powder in the treatment of PUC.Finally,the binding sites and molecular docking were verified with Autodock software.Through data analysis,it was concluded that the treatment of PUC by Guanyu Zhixie Powder may be related to quercetin,matrine,kaempferol,β-sitosterol,naringenin,luteolin,licorice isoflavones and other chemical components.Fos,jun,rela,esr1 and mapk1 were selected as the core targets.Enrichment analysis obtained 2 461 GO entries and 176 KEGG pathways,mainly involving inflammatory signaling pathways and PI3K/Akt signaling pathways.Molecular docking results also showed that the key active ingredients had good binding activity with the corresponding targets.The active components in Guanyu Zhixie Powder can regulate multiple signaling pathways,inhibit inflammatory response and exert the effect of preventing and treating ulcerative colitis in pigs by interfering with key targets such as fos,jun,rela,esr1 and mapk1.

关 键 词：鹳榆止泻散 猪溃疡性结肠炎 网络药理学 分子对接 作用机制 

分 类 号：S859.3[农业科学—临床兽医学]