脂肪来源干细胞移植对脑缺血大鼠脑红蛋白表达的影响及作用机制  

The Effect and Mechanism of Adipose-Derived Stem Cells Transplantation on Expression of Neuroglobin in Rats with Cerebral Ischemia

作　　者：黄欢[1,2] 郑谋炜 梅爱农[1,2] HUANG Huan;ZHENG Mouwei;MEI Ainong(Department of Geriatric Medicine,Fujian Provincial Hospital,Fuzhou 350001,China;Provincial Clinical Medical College of Fujian Medical University,Fuzhou 350001,China)

机构地区：[1]福建省立医院干部特诊一科,福州350001 [2]福建医科大学省立临床医学院,福州350001

出　　处：《中国细胞生物学学报》2024年第10期1755-1763,共9页Chinese Journal of Cell Biology

基　　金：福建省自然科学基金(批准号:2020J011073)资助的课题。

摘　　要：该文探讨了脂肪来源干细胞(ADSC)移植对脑缺血大鼠梗死周边区皮质中脑红蛋白(Ngb)表达的影响,以揭示ADSC促进脑梗死后神经功能恢复的相关机制。SD大鼠采用改良的Longa线栓法建立大脑中动脉闭塞(MCAO)缺血性脑梗死模型,分为假手术组(sham)、模型组(MCAO)和移植组(ADSC)。模型建立后24 h,ADSC组大鼠通过颈动脉途径移植注射50μL的ADSC悬液(含细胞约2×10^(6)个),而MCAO组大鼠则注射相同体积的生理盐水。于模型建立后第1、3、7天采用修正版神经功能缺损评分(mNSS)评估各组大鼠的神经功能。末次m NSS评估后将大鼠断头取脑,采用透射电镜观测脑梗死周边区皮质中线粒体的损伤情况;采用试剂盒检测该区域氧化应激和能量代谢相关指标的水平;采用Western blot法和免疫荧光法检测该区域Ngb的表达情况。结果显示与MCAO组大鼠相比,ADSC移植可显著改善大鼠的m NSS评分,减轻脑梗死周边区皮质的线粒体损伤;显著提高该区域三磷酸腺苷(ATP)、Na^(+)-K^(+)-ATP酶、Ca^(2+)-Mg^(2+)-ATP酶和超氧化物歧化酶(SOD)的水平,以及抑制丙二醛(MDA)、一氧化氮(NO)的水平;并且显著增加神经元细胞胞质中Ngb蛋白的表达水平。总之,ADSC移植可以促进脑缺血大鼠神经功能的恢复,其可能部分通过促进Ngb在梗死周边区皮质中的表达,进而起到保护线粒体结构、改善能量代谢缺陷和减轻氧化应激反应的作用。The objective of this research was to investigate the impact of ADSC(adipose-derived stem cell)transplantation on Ngb(neuroglobin)expression in the peri-infarct cortex of rats with cerebral ischemia,and to elucidate the mechanisms by which ADSC facilitate the recovery of neurological function following cerebral infarction.MCAO(middle cerebral artery occlusion)model was established in SD(Sprague-Dawley)rats using modified Longa’s method,and the animals were randomly assigned to three groups:sham group,MCAO group,and ADSC group.At 24 hours post-model establishment,the ADSC group received intravenous injection of 50μL ADSC suspension(approximately 2×10^(6) cells)via the carotid artery,whereas the MCAO group was administered an equivalent volume of saline.Neurological function was assessed using mNSS(modified neurological severity scores)on the 1st,3rd,and 7th days post-model establishment.Subsequently,the rats were euthanized,and their brains were harvested for analysis.Mitochondrial damage in the peri-infarct cortex was examined using transmission electron microscope,and levels of oxidative stress and energy metabolism indicators were measured using assay kits.The expression of Ngb was determined using Western blot and immunofluorescence techniques.The results indicated that ADSC transplantation significantly improved the mNSS scores of rats,mitigated mitochondrial damage in the peri-infarct cortex,and increased the levels of ATP(adenosine triphosphate),Na^(+)-K^(+)-ATPase,Ca^(2+)-Mg^(2+)-ATPase,and SOD(superoxide dismutase),while also suppressing the levels of MDA(malondialdehyde)and NO(nitric oxide).Furthermore,ADSC transplantation notably elevated the expression of Ngb protein within the cytoplasm of neuronal cells.In summary,ADSC transplantation appears to enhance neurological function in rats post-cerebral infarction,potentially through the upregulation of Ngb expression in the peri-infarct cortex,which may protect mitochondrial structure,ameliorate energy metabolism deficiencies,and diminish the oxidative

关 键 词：脑梗死 干细胞 脑红蛋白 线粒体 氧化应激 能量代谢 

分 类 号：R743.3[医药卫生—神经病学与精神病学]