荷叶中生物碱类化合物及其降血脂活性研究  

Alkaloid compounds in Nelumbinis Folium and their hypolipidemic effect

作　　者：江健 许倩楠 车莉 赖志成 田文静[1] 陈海峰[1] JIANG Jian;XU Qian-nan;CHE Li;LAI Zhi-cheng;TIAN Wen-jing;CHEN Hai-feng(School of Pharmaceutical Sciences,Xiamen University,Xiamen 361000,China;Xiamen Key Laboratory of Research and Development of Proprietary Chinese Medicine,Xiamen Traditional Chinese Medicine Co.,Ltd.,Xiamen 361000,China)

机构地区：[1]厦门大学药学院,福建厦门361000 [2]厦门中药厂有限公司厦门市中成药研究与开发重点实验室,福建厦门361000

出　　处：《中国中药杂志》2024年第19期5238-5243,共6页China Journal of Chinese Materia Medica

基　　金：厦门市科技计划项目(3502Z20224017)。

摘　　要：运用硅胶柱色谱、ODS柱色谱和制备型高效液相色谱等方法从荷叶的70%乙醇提取物中分离提取得到4个生物碱类化合物,包括1个新化合物和3个已知化合物。利用核磁共振波谱、高分辨质谱、圆二色谱等方法对其进行结构鉴定,其中化合物1为新化合物,命名为lotustine B(1)。化合物2~4为已知化合物,分别鉴定为nuciferin(2)、dehydronuciferine(3)、N-methylasimilobine(4)。通过构建HepG2细胞高血脂模型,评价了分离得到的4个生物碱类化合物对总胆固醇(TC)及总甘油三酯(TG)水平的影响,同时也利用油红O染色的实验方法评价了化合物1~4的降血脂活性,结果显示化合物4能降低HepG2高脂模型细胞内的脂质累积,具有潜在的降血脂活性。Four alkaloid compounds including one new compound and three known ones were isolated and extracted from the 70%ethanol extract of Nelumbinis Folium by silica gel column chromatography,octadecylsilyl(ODS)column chromatography and prepara-tive high-performance liquid chromatography.Their structures were identified by nuclear magnetic resonance spectroscopy,high-resolu-tion mass spectrometry,circular dichroism and other methods.Compound 1 was identified as a new compound and named as lotustine B(1).Compounds 2-4 were identified as nuciferin(2),dehydronuciferine(3),N-methylasimilobine(4).The effects of four isola-ted alkaloid compounds on total cholesterol(TC)and total triglycerides(TG)levels were evaluated by constructing a human hepatoma(HepG2)cell model of hyperlipidemia.The hypolipidemic effects of compounds 1-4 were also evaluated using oil red O staining experi-ments.The results showed that compound 4 can reduce the lipid levels of HepG2 cells and has potential hypolipidemic effects.

关 键 词：荷叶 生物碱类化合物 降血脂活性 

分 类 号：R285[医药卫生—中药学]