Mapping the Behavioral Signatures of Shank3b Mice in Both Sexes  

作　　者：Jingjing Liu Jialin Ye Chunyuan Ji Wenting Ren Youwei He Fuqiang Xu Feng Wang 

机构地区：[1]NMPA Key Laboratory for Research and Evaluation of Viral Vector Technology in Cell and Gene Therapy Medicinal Products,Shenzhen-Hong Kong Institute of Brain Science,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,518055,China [2]Shenzhen Key Laboratory of Viral Vectors for Biomedicine,Translational Research Center for the Nervous System,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,518055,China [3]Key Laboratory of Quality Control Technology for Virus-Based Therapeutics,Guangdong Provincial Medical Products Administration,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,518055,China [4]Shenzhen Key Lab of Translational Research for Brain Diseases,Translational Research Center for the Nervous System,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,518055,China [5]Guangdong Provincial Key Laboratory of Brain Connectome and Behavior,the Brain Cognition and Brain Disease Institute,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,518055,China [6]CAS Key Laboratory of Brain Connectome and Manipulation,the Brain Cognition and Brain Disease Institute,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,518055,China [7]University of Chinese Academy of Sciences,Beijing,100049,China

出　　处：《Neuroscience Bulletin》2024年第9期1299-1314,共16页神经科学通报（英文版）

基　　金：National Science and Technology Innovation 2030-Major Project of China(2021ZD0201003 and 2022ZD0208300);National Natural Science Foundation of China(32171092);Shenzhen Key Basic Research Project(JCYJ20220818100805013);Shenzhen Key Laboratory of Viral Vectors for Biomedicine(ZDSYS20200811142401005);Guangdong Provincial Medical Products Administration Key Laboratory of Quality Control Technology for Virus-Based Therapeutics(2020ZDB26);Guangdong Provincial Medical Products Administration(2022ZDZ13).

摘　　要：Autism spectrum disorders(ASD)are characterized by social and repetitive abnormalities.Although the ASD mouse model with Shank3b mutations is widely used in ASD research,the behavioral phenotype of this model has not been fully elucidated.Here,a 3D-motion capture system and linear discriminant analysis were used to comprehensively record and analyze the behavioral patterns of male and female Shank3b mutant mice.It was found that both sexes replicated the core and accompanied symptoms of ASD,with significant sex differences.Further,Shank3b heterozygous knockout mice exhibited distinct autistic behaviors,that were significantly different from those those observed in the wild type and homozygous knockout groups.Our findings provide evidence for the inclusion of both sexes and experimental approaches to efficiently characterize heterozygous transgenic models,which are more clinically relevant in autistic studies.

关 键 词：AUTISM Shank3b Spontaneous behavior 3D animal motion-capture system Computational ethology Sex differences 

分 类 号：R749[医药卫生—神经病学与精神病学]